Zhao Jing, Li Qian, Hao Xuefang, Ren Xiangkui, Guo Jintang, Feng Yakai, Shi Changcan
School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China.
J Mater Chem B. 2017 Oct 28;5(40):8035-8051. doi: 10.1039/c7tb02012k. Epub 2017 Oct 4.
Non-viral gene carriers for gene therapy have been developed for many years. But the gene transfection is generally limited by deficient cellular uptake, low endo/lysosome escape, and weak nuclear translocation. Some targeting peptides have been conjugated onto gene carriers for highly efficient gene delivery. These targeting carriers can overcome some of these limitations to efficiently deliver therapeutic genes into desired cells. In this review, we will summarize the recent development of multi-targeting peptide immobilized non-viral gene carriers for efficient gene therapy, especially for the targeting and suppression of tumor cells, and the transfection and proliferation of endothelial cells. The peptide functionalization of gene carriers is a promising strategy to promote the elimination of solid tumors and the rapid endothelialization of artificial blood vessels.
用于基因治疗的非病毒基因载体已经研发多年。但基因转染通常受到细胞摄取不足、内吞/溶酶体逃逸率低和核转位能力弱的限制。一些靶向肽已被连接到基因载体上以实现高效基因递送。这些靶向载体可以克服其中一些限制，从而有效地将治疗性基因递送至所需细胞。在本综述中，我们将总结用于高效基因治疗的多靶向肽固定化非病毒基因载体的最新进展，特别是针对肿瘤细胞的靶向和抑制以及内皮细胞的转染和增殖。基因载体的肽功能化是促进实体瘤消除和人工血管快速内皮化的一种有前景的策略。
