Laboratory of Criminalistics, Adam Mickiewicz University, Św. Marcin 90, 61-809, Poznań, Poland.
Centre for Advanced Technologies, Adam Mickiewicz University, Uniwersytetu Poznańskiego 10, 61-614, Poznań, Poland.
Int J Legal Med. 2020 Jul;134(4):1531-1545. doi: 10.1007/s00414-020-02275-3. Epub 2020 Apr 8.
The hairy rove beetle, Creophilus maxillosus (Linnaeus) (Staphylinidae), is recognized for its use in forensic entomology. However, insufficient developmental data exist for the Central European population of this species. Accordingly, we studied the development of C. maxillosus at ten constant temperatures (10-32.5 °C). Based on these results, linear and nonlinear developmental models were created and validated. We also studied the effect of different homogenous diets (third-instar larvae or puparia of Calliphora sp. Robineau-Desvoidy or Lucilia sp. Robineau-Desvoidy (Diptera: Calliphoridae) or mix of first- and second-instar larvae of Necrodes littoralis (Linnaeus) (Coleoptera: Silphidae)) on the development and mortality of C. maxillosus. Average total development times ranged between 122.21 days at 15 °C and 22.18 days at 30 °C. Beetles reached the adult stage in seven out of ten temperatures (15-30 °C). No beetles reached the adult stage when fed with larvae of N. littoralis; their development times at first and second larval stage were also significantly longer than in other food conditions. When C. maxillosus larvae were fed with blowfly larvae, the highest mortality was observed at the pupal stage, as compared when they were fed with blowfly puparia-at the first larval stage. While validating thermal summation models, the highest age estimation errors were found for beetles bred at 10 and 12.5 °C (between 21 and 43% for all developmental events). Age estimation errors were on average higher for pupation and eclosion than hatching and first and second ecdyses. While validating the models with specimens fed with different diets, the highest errors were recorded for beetles fed with N. littoralis larvae (22% for the first ecdysis and 33% for the second ecdysis) and Lucilia sp. puparia (32% for pupation and 22% for eclosion). Implications for C. maxillosus use in forensic entomology are discussed.
多毛长足隐翅虫，Creophilus maxillosus (Linnaeus)（隐翅虫科），因其在法医昆虫学中的应用而被认可。然而，这种物种在中欧种群的发育数据不足。因此，我们研究了 C. maxillosus 在十个恒温（10-32.5°C）下的发育情况。根据这些结果，创建和验证了线性和非线性发育模型。我们还研究了不同同质饮食（三龄幼虫或丽蝇蛹或绿蝇蛹（双翅目：丽蝇科）或混合第一和第二龄幼虫 Necrodes littoralis (Linnaeus)（鞘翅目：埋葬甲科））对 C. maxillosus 发育和死亡率的影响。平均总发育时间在 15°C 时为 122.21 天，在 30°C 时为 22.18 天。在十种温度中的七种温度下，甲虫达到成虫阶段（15-30°C）。当喂食 N. littoralis 幼虫时，没有甲虫达到成虫阶段；它们在第一和第二幼虫阶段的发育时间也明显长于其他食物条件。当 C. maxillosus 幼虫喂食蝇幼虫时，在蛹期观察到最高的死亡率，而当它们喂食蝇蛹时，在第一幼虫期观察到最高的死亡率。在验证热总和模型时，在 10 和 12.5°C 下饲养的甲虫发现年龄估计误差最高（所有发育事件的误差为 21%至 43%）。在孵化、第一和第二蜕皮时，蛹化和出眠的年龄估计误差平均高于出眠和第一和第二蜕皮。在用不同饮食喂养的标本验证模型时，喂食 N. littoralis 幼虫的甲虫记录的误差最高（第一次蜕皮 22%，第二次蜕皮 33%）和 Lucilia sp. 蛹（蛹化 32%，出眠 22%）。讨论了 C. maxillosus 在法医昆虫学中的应用的影响。
