Fernández-Calvo Patricia, Iñigo Sabrina, Glauser Gaétan, Vanden Bossche Robin, Tang Michelle, Li Baohua, De Clercq Rebecca, Nagels Durand Astrid, Eeckhout Dominique, Gevaert Kris, De Jaeger Geert, Brady Siobhan M, Kliebenstein Daniel J, Pauwels Laurens, Goossens Alain, Ritter Andrés
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, B-9052, Ghent, Belgium.
VIB Center for Plant Systems Biology, Technologiepark 71, B-9052, Ghent, Belgium.
New Phytol. 2020 Aug;227(4):1124-1137. doi: 10.1111/nph.16586. Epub 2020 May 14.
The sessile lifestyle of plants requires accurate physiology adjustments to be able to thrive in a changing environment. Plants integrate environmental timing signals to control developmental and stress responses. Here, we identified Far1 Related Sequence (FRS) 7 and FRS12, two transcriptional repressors that accumulate in short-day conditions, as regulators of Arabidopsis glucosinolate (GSL) biosynthesis. Loss of function of FRS7 and FRS12 results in plants with increased amplitudes of diurnal expression of GSL pathway genes. Protein interaction analyses revealed that FRS7 and FRS12 recruit the NOVEL INTERACTOR OF JAZ (NINJA) to assemble a transcriptional repressor complex. Genetic and molecular evidence demonstrated that FRS7, FRS12 and NINJA jointly regulate the expression of GSL biosynthetic genes, and thus constitute a molecular mechanism that modulates specialized metabolite accumulation.
植物固着的生活方式需要精确的生理调节，以便能够在不断变化的环境中茁壮成长。植物整合环境时间信号来控制发育和应激反应。在这里，我们鉴定出Far1相关序列（FRS）7和FRS12，这两个在短日照条件下积累的转录抑制因子，作为拟南芥硫代葡萄糖苷（GSL）生物合成的调节因子。FRS7和FRS12功能缺失导致GSL途径基因昼夜表达幅度增加的植株。蛋白质相互作用分析表明，FRS7和FRS12招募JAZ的新型相互作用蛋白（NINJA）来组装转录抑制复合物。遗传和分子证据表明，FRS7、FRS12和NINJA共同调节GSL生物合成基因的表达，从而构成一种调节特殊代谢物积累的分子机制。
