日本根际荧光假单胞菌中具有抗生素生物合成基因的多样性及其生物防治功效。
Diversity of Antibiotic Biosynthesis Gene-possessing Rhizospheric Fluorescent Pseudomonads in Japan and Their Biocontrol Efficacy.
机构信息
Institute of Vegetable and Floriculture Science, National Agriculture and Food Research Organization (NARO).
Institute of Agrobiological Sciences, NARO.
出版信息
Microbes Environ. 2020;35(2). doi: 10.1264/jsme2.ME19155.
More than 3,000 isolates of fluorescent pseudomonads have been collected from plant roots in Japan and screened for the presence of antibiotic-synthesizing genes. In total, 927 hydrogen cyanide (HCN)-, 47 2,4-diacetylphloroglucinol (PHL)-, 6 pyoluteorin (PLT)-, 14 pyrrolnitrin (PRN)-, and 8 phenazine (PHZ)-producing isolates have been detected. A cluster analysis (≥99% identity) identified 10 operational taxonomic units (OTUs) in antibiotic biosynthesis gene-possessing pseudomonads. OTU HLR (PHL, PLT, and PRN) contained four antibiotics: HCN, PHL, PLT, and PRN, while OTU RZ (PRN and PHZ) contained three: HCN, PRN, and PHZ. OTU H1, H2, H3, H4, H5, H6, and H7 (PHL1-7) contained two antibiotics: HCN and PHL, while OTU H8 (PHL8) contained one: PHL. Isolates belonging to OTU HLR and RZ suppressed damping-off disease in cabbage seedlings caused by Rhizoctonia solani. Effective strains belonging to OTU HLR and RZ were related to Pseudomonas protegens and Pseudomonas chlororaphis, respectively. Antibiotic biosynthesis gene-possessing fluorescent pseudomonads are distributed among different geographical sites in Japan and plant species.
从日本植物根系中收集了 3000 多个荧光假单胞菌分离株，并对其抗生素合成基因的存在进行了筛选。共检测到 927 株产生氢氰酸（HCN）、47 株产生 2,4-二乙酰基间苯三酚（PHL）、6 株产生吡咯并[1,2-a]嘧啶-2,4-二酮（PLT）、14 株产生吡咯并[1,2-b]哒嗪-3,4-二酮（PRN）和 8 株产生吩嗪（PHZ）的产生菌。聚类分析（≥99%的同一性）确定了具有抗生素生物合成基因的假单胞菌中的 10 个操作分类单元（OTU）。OTU HLR（PHL、PLT 和 PRN）含有 4 种抗生素：HCN、PHL、PLT 和 PRN，而 OTU RZ（PRN 和 PHZ）含有 3 种：HCN、PRN 和 PHZ。OTU H1、H2、H3、H4、H5、H6 和 H7（PHL1-7）含有 2 种抗生素：HCN 和 PHL，而 OTU H8（PHL8）含有 1 种：PHL。属于 OTU HLR 和 RZ 的分离株可抑制由立枯丝核菌引起的白菜幼苗猝倒病。属于 OTU HLR 和 RZ 的有效菌株分别与荧光假单胞菌和假单胞菌有关。具有抗生素生物合成基因的荧光假单胞菌分布在日本不同的地理地点和植物物种中。
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