Stangier J, Hilbich C, Dircksen H, Keller R
Institut für Zoophysiologie, Rheinische Friedrich Wilhelms-Universität, Bonn, Federal Republic of Germany.
Peptides. 1988 Jul-Aug;9(4):795-800. doi: 10.1016/0196-9781(88)90124-6.
A radioimmunoassay (RIA) for the recently discovered crustacean cardioactive peptide (CCAP) has been developed and used to determine contents of CCAP in different parts of the nervous system of the shore crab Carcinus maenas. Immunoreactive material was detected throughout the nervous system. In contrast to the main ganglia which contained low levels of approximately 1.4 pmol CCAP/mg protein (brain and thoracic ganglion), a high concentration was found in a neurohemal structure, the pericardial organs (PO) (868 pmol/mg protein). A predominantly neurohormonal role of CCAP thus suggested is further supported by in vitro release studies. Incubation of POs in high (K+) saline showed that CCAP is secretable in considerable amounts by a Ca++-dependent release mechanism.
已开发出一种针对最近发现的甲壳类心脏活性肽（CCAP）的放射免疫分析法（RIA），并用于测定滨蟹（Carcinus maenas）神经系统不同部位的CCAP含量。在整个神经系统中均检测到免疫反应性物质。与主要神经节（脑和胸神经节）中CCAP含量较低（约1.4 pmol/mg蛋白质）形成对比的是，在一种神经血器官——围心器官（PO）中发现了高浓度的CCAP（868 pmol/mg蛋白质）。体外释放研究进一步支持了由此提出的CCAP主要起神经激素作用的观点。将POs置于高（钾离子）盐溶液中孵育表明，CCAP可通过钙离子依赖的释放机制大量分泌。
