Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Key Laboratory for Tobacco Gene Resources, State Tobacco Monopoly Administration, Qingdao, 266101, China.
Mol Biotechnol. 2020 Aug;62(8):364-369. doi: 10.1007/s12033-020-00252-0.
Targeted gene expression in plants allows us to further study biological traits of interest, such as reproductive and developmental processes. Here, the tobacco TA29 anther-specific promoter was used to direct the expression of the ricin enzymatic subunit A (RTA) in transgenic tobacco plants, phenotypic analysis of the resulting positive transgenic tobacco (Nicotiana tabacum L.) plants demonstrated that RTA expression led to a reduction in pistil length and shriveling of anthers, as well as the grayish-brown color of anthers, the reduced pollen viability and male sterility. For the first time, a plant-derived ricin gene enzymatic subunit A (RTA) expression system under the tissue-specific promoter was demonstrated to be sensitive and efficient in controlling plant sterility and creating male-sterile materials. Consequently, it could be used to control other agronomic traits and produce hybrid seeds in plants in the future.
目的基因在植物中的表达使我们能够进一步研究感兴趣的生物学特性，如生殖和发育过程。在这里，我们使用烟草 TA29 花药特异性启动子来指导蓖麻毒素酶亚基 A（RTA）在转基因烟草植物中的表达，对产生的阳性转基因烟草（Nicotiana tabacum L.）植株的表型分析表明，RTA 的表达导致雌蕊长度缩短、花药干瘪以及花药呈灰棕色，花粉活力和雄性可育性降低。首次证明，在组织特异性启动子下，植物来源的蓖麻毒素基因酶亚基 A（RTA）表达系统在控制植物不育和创造雄性不育材料方面具有敏感性和高效性。因此，它可以用于控制其他农艺性状，并在未来的植物中生产杂交种子。
