Department of Chemical Engineering and Interagency Convergence Energy on New Biomass Industry, Hankyong National University, 327, Jungang-ro, Anseong-si, Gyeonggi-do, 17579, South Korea.
Appl Biochem Biotechnol. 2020 Sep;192(1):103-119. doi: 10.1007/s12010-020-03311-z. Epub 2020 Apr 8.
The objectives of our research are to investigate the concept of delignification from pinecone through alkaline fractionation and then extraction of formic acid from the hydrolysate through esterification using ethanol. The pinecone is considered a promising material because of its relatively higher lignin content (35.80%) than other lignocellulosic biomass. The recovery yield of acid insoluble lignin (AIL) reached its maximum value of 79.20% at 8% NaOH, and the concentration of formic acid in the hydrolysate had its highest value under the same conditions. Moreover, the glucan content in fractionated solid remained high. The hydrolysate was subjected to esterification with ethanol under various reaction conditions for formic acid extraction, with solvent mixing ratio range: 1:1-1:4 v/v, reaction temperature range: 30-45 °C, and reaction time range: 60-100 min. Subsequently, the ethanol mixture (ethanol and ethyl formate) was recovered through distillation. The formic acid was extracted with more than 85% at mixing ratio of 1:2 and 45 °C for all reaction times. Furthermore, salt compounds composed mainly of Na and S were recovered because of its properties not soluble in ethanol solution.
我们研究的目的是通过碱性分馏从松果中提取木质素，然后通过酯化作用用乙醇从水解产物中提取甲酸。松果因其相对较高的木质素含量（35.80%）而被认为是一种很有前途的材料，比其他木质纤维素生物质都高。在 8%NaOH 条件下，酸不溶性木质素（AIL）的回收率达到最大值 79.20%，在相同条件下，水解产物中甲酸的浓度达到最高值。此外，分馏后的固体中的葡聚糖含量仍然很高。在不同的反应条件下，将水解产物与乙醇进行酯化反应以提取甲酸，溶剂混合比范围为 1:1-1:4v/v，反应温度范围为 30-45°C，反应时间范围为 60-100min。随后，通过蒸馏回收乙醇混合物（乙醇和甲酸乙酯）。在混合比为 1:2 和 45°C 下，所有反应时间的甲酸提取率均超过 85%。此外，由于其不溶于乙醇溶液的性质，主要由 Na 和 S 组成的盐化合物得到了回收。
