College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang 330045, China.
College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang 330045, China.
Bioresour Technol. 2022 Sep;359:127502. doi: 10.1016/j.biortech.2022.127502. Epub 2022 Jun 17.
A choline chloride-formic acid (ChCl-FA) pretreatment followed by enzymatic hydrolysis and fermentation were developed in this work for co-produce bioethanol, xylose, and lignin from eucalyptus. Results showed that ChCl-FA pretreatment can simultaneously degrade the xylan (∼95.2%) and lignin (∼74.4%) in eucalyptus, and obtained the pretreated eucalyptus having high glucan content and a numbers of cracks and holes, which was conducive to follow-up cellulase attacking. The hydrolysis experiments showed the maximum yield of glucose of 100 g eucalyptus was 35.3 g, which was equivalent to 90.3% of glucan in eucalyptus feedstock. The fermentation of enzymatic hydrolysate finally achieved the ethanol yield of 16.5 g, which corresponded to 74.5% theoretical ethanol yield from initial glucan in eucalyptus. In addition, 12.1 g xylose and 23.9 g lignin also could be obtained in pretreated liquid or/and hydrolysis residue, which represented for 61.4% xylan and 80.7% lignin in eucalyptus feedstock, respectively.
本工作采用氯化胆碱-甲酸（ChCl-FA）预处理，然后进行酶解和发酵，从桉树中共同生产生物乙醇、木糖和木质素。结果表明，ChCl-FA 预处理可以同时降解桉树中的木聚糖（95.2%）和木质素（74.4%），并获得预处理后的桉树具有较高的葡聚糖含量和许多裂缝和孔，有利于后续纤维素酶的攻击。水解实验表明，最大葡萄糖得率为 100 g 桉树 35.3 g，相当于桉树原料中葡聚糖的 90.3%。酶解液的发酵最终实现了 16.5 g 的乙醇产量，相当于初始葡聚糖理论乙醇产率的 74.5%。此外，在预处理液或/和水解残渣中还可以得到 12.1 g 木糖和 23.9 g 木质素，分别代表桉树原料中木聚糖的 61.4%和木质素的 80.7%。
