Tarbiat Modares University Faculty of Medical Sciences Tehran, Tehran, Islamic Republic of Iran.
Daru. 2020 Jun;28(1):209-219. doi: 10.1007/s40199-020-00331-2. Epub 2020 Apr 8.
Many solutions have been evaluated to deal with "chemotherapy and radiation-resistant cancer cells' as well as "severe complications of chemotherapy drugs". One of these solutions is the use of herbal compounds with antioxidant properties. Among these antioxidant compounds, curcumin is identified as the strongest one to inhibit cancerous cells proliferation. However, its clinical trials have encountered many constraints, because curcumin is insoluble in water and unstable in physiological conditions. To overcome these limitations, in this study, curcumin was conjugated with human serum albumin (HSA) and its effects on breast cancer cell lines were also measured.
After making of HSA-curcumin nanoparticles (NPs) by the desolvation technique, they were characterized by the FTIR, DLS, TEM, and SEM method. At the end, its anticancer effects have been examined using MTT test and apoptosis assay.
The FTIR graph confirmed that curcumin and HSA have been conjugated along with each other. Particles size was reported to be 220 nm and 180 nm by DLS and SEM, respectively. The zeta potential of HSA-curcumin NPs was -7 mV, while it was -37 mV for curcumin. The MTT and apoptosis assay results indicated that the toxicity of HSA-curcumin NPs on the normal cell are less than curcumin; however, its anti-cancer effects on the cancer cells are much greater, compared to curcumin.
HSA-curcumin NPs increase curcumin solubility in water as well as its stability in physiological and acidic conditions. These factors have the ability of overwhelming the limitations on using curcumin alone, and they could result in a significant increase in the toxicity of curcumin on the cancer cells without increasing its toxicity on the normal cells. Grapical abstract.
许多解决方案已被评估用于处理“化疗和放疗耐药癌细胞”以及“化疗药物的严重并发症”。其中一种解决方案是使用具有抗氧化特性的草药化合物。在这些抗氧化化合物中，姜黄素被确定为最强的抑制癌细胞增殖的化合物。然而，其临床试验遇到了许多限制，因为姜黄素不溶于水且在生理条件下不稳定。为了克服这些限制，在这项研究中，姜黄素与人体血清白蛋白（HSA）结合，并测量其对乳腺癌细胞系的影响。
通过去溶剂化技术制备 HSA-姜黄素纳米粒子（NPs）后，通过傅里叶变换红外光谱（FTIR）、动态光散射（DLS）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）对其进行了表征。最后，通过 MTT 试验和凋亡试验检测其抗癌作用。
FTIR 图谱证实姜黄素和 HSA 已相互结合。DLS 和 SEM 分别报道粒径为 220nm 和 180nm。HSA-姜黄素 NPs 的 zeta 电位为-7mV，而姜黄素的 zeta 电位为-37mV。MTT 和凋亡试验结果表明，HSA-姜黄素 NPs 对正常细胞的毒性小于姜黄素；然而，与姜黄素相比，其对癌细胞的抗癌作用要大得多。
HSA-姜黄素 NPs 提高了姜黄素在水中的溶解度及其在生理和酸性条件下的稳定性。这些因素克服了单独使用姜黄素的限制，使姜黄素对癌细胞的毒性显著增加，而对正常细胞的毒性没有增加。
