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一种用于检测抗禽腺病毒7型和8型抗体的高效基于纤维的酶联免疫吸附测定法。

An efficient fiber-based ELISA for detection of antibody against fowl adenovirus serotypes 7 and 8.

作者信息

Lu Hao, Wang Weikang, Zhang Jianjun, Shao Hongxia, Li Luyuan, Li Tuofan, Xie Quan, Wan Zhimin, Qin Aijian, Ye Jianqiang

机构信息

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine; and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China; and Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China (Lu, Wang, Shao, L. Li, T. Li, Xie, Wan, Qin, Ye).

Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China (Lu, Wang, Shao, L. Li, T. Li, Xie, Wan, Qin, Ye).

出版信息

J Vet Diagn Invest. 2020 May;32(3):444-449. doi: 10.1177/1040638720913354. Epub 2020 Apr 9.

Abstract

An outbreak of inclusion body hepatitis caused by fowl adenovirus serotype 8 (FAdV-8) has caused significant economic losses in the poultry industry worldwide. However, a rapid serology test kit specific to FAdV-8 is not available to date. We developed a fiber-based ELISA using the purified GST-fiber of FAdV-8 as coating antigen to measure antibodies against FAdV-8. Specificity analysis showed that our ELISA could react with sera against FAdV-7, -8a, and -8b, but not with sera against the other pathogens tested. Moreover, detection of positive sera with our ELISA had 83% and 94% agreement with an indirect immunofluorescence assay (IFA) and a commercial ELISA from BioChek, respectively. Our ELISA was also effective in the detection of antibodies against FAdV-8 in sera from both experimentally infected and clinically vaccinated chickens. Our FAdV-8 fiber-based ELISA can be a valuable tool to specifically and sensitively detect antibodies against FAdV-7 and/or -8 in infected or vaccinated chickens.

摘要

由8型禽腺病毒(FAdV-8)引起的包涵体肝炎暴发已给全球家禽业造成了重大经济损失。然而,迄今为止尚无针对FAdV-8的快速血清学检测试剂盒。我们开发了一种基于纤维的酶联免疫吸附测定(ELISA),使用纯化的FAdV-8 GST-纤维作为包被抗原,以检测抗FAdV-8抗体。特异性分析表明,我们的ELISA可与抗FAdV-7、-8a和-8b的血清发生反应,但不与针对其他检测病原体的血清发生反应。此外,用我们的ELISA检测阳性血清与间接免疫荧光测定(IFA)以及BioChek公司的商业ELISA的一致性分别为83%和94%。我们的ELISA在检测实验感染鸡和临床接种鸡血清中的抗FAdV-8抗体方面也很有效。我们基于FAdV-8纤维的ELISA可以成为一种有价值的工具,用于特异性和灵敏地检测感染或接种鸡中抗FAdV-7和/或-8的抗体。

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