活性氧物种检测中 ABTS 法的实验步骤及优化方案。
Protocol of proceedings with and optimization of ABTS method for detection of reactive oxygen species.
机构信息
Department of Microbiology, Institute of Genetics & Microbiology, University of Wrocław, S. Przybyszewskiego 63, Wrocław 50-001, Poland.
Faculty of Chemistry, University of Wrocław, F. Joliot-Curie 14, Wrocław 50-383, Poland.
出版信息
Future Microbiol. 2020 Mar;15:259-271. doi: 10.2217/fmb-2019-0010.
Characterization of the ability of DSM 15643 and DSM 20482 strains in the presence of Cu and HO to reactive oxygen species generation. Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used. Determination of: MIC for Cu, HO and ABTS; survivability of under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria. in the presence of Cu and HO is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process.
研究 DSM 15643 和 DSM 20482 菌株在 Cu 和 HO 存在下产生活性氧物质的能力。采用分光光度 ABTS（2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸））法。测定：Cu、HO 和 ABTS 的 MIC；在大气氧暴露下的生存能力；细菌产生自由基的水平和速率常数。在 Cu 和 HO 的存在下能够产生自由基。活性氧主要在细菌细胞外产生，这表明外膜蛋白可能参与氧化过程。
Zotero 插件