Department of Gynaecology and Obstetrics, Cangzhou Central Hospital, Cangzhou City, Hebei Province, China.
Eur Rev Med Pharmacol Sci. 2020 Mar;24(6):2928-2937. doi: 10.26355/eurrev_202003_20656.
Several long noncoding RNAs (lncRNAs) display functional effects in the tumorigenesis and progression of cervical cancer (CC). We aimed to investigate the roles of lncRNA tyrosine protein kinase transmembrane receptor 1 antisense RNA 1 (ROR1‑AS1) in the development of CC patients.
Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was performed for the determination of ROR1‑AS1 levels in both CC tissues and cell lines. The clinical value of ROR1‑AS1 expression in CC patients was statistically analyzed. After transfection with si-ROR1‑AS1 in SiHa and HeLa cells, cellular growth and apoptosis were examined by Cell Counting Kit (CCK-8) assay, cell colony formation, and flow cytometry. Then, wound-healing assays and transwell assays were performed to evaluate cell migration and invasion, respectively. The related proteins of epithelial-mesenchymal transition (EMT) markers and Wnt/β-catenin signaling pathway was assessed using Western blot assays.
We found that that the expressions of ROR1‑AS1 were distinctly increased in CC tissues and cell lines. Clinical study revealed that high ROR1‑AS1 expression was associated with distant metastasis, FIGO stage, and shorter five-year survival. Functional assays by performing in vitro assays revealed that inhibition of ROR1‑AS1 distinctly suppressed CC cell proliferation, colony formation, migration and invasion, and promoted apoptosis. Based on results of Western blot, we showed that the downregulation of ROR1‑AS1 inhibited the levels of N-cadherin and vimentin. In addition, the distinctly decreased levels of c-myc, β-catenin, and cyclin D1 were observed in CC cells transfected with si-ROR1‑AS1.
Our results suggest that ROR1‑AS1 is likely to serve as an efficient therapeutic approach in respect of CC treatment. Our results suggest that KLF5 may be a potential therapeutic target in laryngeal carcinoma.
几种长链非编码 RNA（lncRNA）在宫颈癌（CC）的发生和发展中表现出功能效应。我们旨在研究酪氨酸蛋白激酶跨膜受体 1 反义 RNA 1（ROR1-AS1）lncRNA 在 CC 患者发展中的作用。
采用逆转录-聚合酶链反应（RT-PCR）测定 CC 组织和细胞系中 ROR1-AS1 的水平。统计分析 ROR1-AS1 在 CC 患者中的临床价值。在 SiHa 和 HeLa 细胞中转染 si-ROR1-AS1 后，通过细胞计数试剂盒（CCK-8）测定、细胞集落形成和流式细胞术检测细胞生长和凋亡。然后，通过划痕愈合实验和 Transwell 实验分别评估细胞迁移和侵袭。采用 Western blot 实验检测上皮-间充质转化（EMT）标志物和 Wnt/β-catenin 信号通路的相关蛋白。
我们发现 ROR1-AS1 在 CC 组织和细胞系中的表达明显增加。临床研究表明，高 ROR1-AS1 表达与远处转移、FIGO 分期和五年生存率较短有关。通过体外实验进行的功能研究表明，抑制 ROR1-AS1 明显抑制 CC 细胞增殖、集落形成、迁移和侵袭，并促进凋亡。基于 Western blot 的结果，我们表明下调 ROR1-AS1 抑制了 N-钙粘蛋白和波形蛋白的水平。此外，在转染 si-ROR1-AS1 的 CC 细胞中，c-myc、β-catenin 和 cyclin D1 的水平明显降低。
我们的研究结果表明，ROR1-AS1 可能成为 CC 治疗的有效治疗方法。我们的研究结果表明，KLF5 可能是喉癌的潜在治疗靶点。
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