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ROR1-AS1 可能促进胆管癌细胞的体内和体外增殖和侵袭。

ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells.

机构信息

Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province, 266003, China.

Department of Hepatobiliary and Pancreatic Surgery, HuiKang Hospital of the Affiliated Hospital of Qingdao University of Qingdao, Qingdao City, Shandong Province, 266520, China.

出版信息

BMC Cancer. 2023 Sep 28;23(1):912. doi: 10.1186/s12885-023-11412-1.

DOI:10.1186/s12885-023-11412-1
PMID:37770853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10536779/
Abstract

Long non-coding RNAs (lncRNAs) play important roles in many pathophysiological processes, including cancer progression. Namely, lncRNA Receptor-tyrosine-kinase-like orphan receptor-1 antisense 1 (ROR1-AS1) is crucial for cancer occurrence and progression in organs such as the liver or bladder. However, its expression and role in cholangiocarcinoma (CCA) have not been thoroughly explored.Firstly, we assessed cell viability, proliferation, invasion, and migration using three cell lines (HuCCT-1, QBC399, and RBE) to explore the biological characteristics of ROR1-AS1 in CCA. Secondly, to determine the in vivo effect of ROR1-AS1 on tumor growth, ROR1-AS1 knockdown (KD) HuCCT-1 cells were subcutaneously injected into nude mice to evaluate tumor growth. Finally, we conducted a bioinformatic analysis to confirm the role of ROR1-AS1 in the prognosis and immunity of CCA.In this study, we found that lncRNA ROR1-AS1 was increased in CCA samples and patients with higher ROR1-AS1 expression had a shorter overall survival period. siRNA-mediated KD of ROR1-AS1 significantly reduced cell proliferation and inhibited the migration of CCA cells. In addition, ROR1-AS1 KD HuCCT-1 cells injected into nude mice grew slower than normal CCA cells.In summary, our results show that ROR1-AS1 can promote CCA progression and might serve as a new target for diagnosis and treatment of CCA.

摘要

长链非编码 RNA（lncRNA）在许多病理生理过程中发挥着重要作用，包括癌症的进展。具体来说，lncRNA 受体酪氨酸激酶样孤儿受体 1 反义 1（ROR1-AS1）对于肝脏或膀胱等器官中的癌症发生和进展至关重要。然而，其在胆管癌（CCA）中的表达和作用尚未得到深入探讨。

首先，我们使用三种细胞系（HuCCT-1、QBC399 和 RBE）评估细胞活力、增殖、侵袭和迁移，以探索 ROR1-AS1 在 CCA 中的生物学特性。其次，为了确定 ROR1-AS1 对肿瘤生长的体内影响，将 ROR1-AS1 敲低（KD）HuCCT-1 细胞皮下注射到裸鼠中以评估肿瘤生长。最后，我们进行了生物信息学分析，以确认 ROR1-AS1 在 CCA 预后和免疫中的作用。

在这项研究中，我们发现 lncRNA ROR1-AS1 在 CCA 样本和高 ROR1-AS1 表达的患者中增加，并且具有更高 ROR1-AS1 表达的患者总体生存期更短。ROR1-AS1 的 siRNA 介导的 KD 显著降低了 CCA 细胞的增殖并抑制了其迁移。此外，注射到裸鼠中的 ROR1-AS1 KD HuCCT-1 细胞的生长速度比正常 CCA 细胞慢。

总之，我们的结果表明 ROR1-AS1 可以促进 CCA 的进展，并且可能成为 CCA 诊断和治疗的新靶标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/0ef5cb6f3117/12885_2023_11412_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/5a031e9f0f7a/12885_2023_11412_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/855fec73ed44/12885_2023_11412_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/ba813ffcb371/12885_2023_11412_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/7ea5ef6825c6/12885_2023_11412_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/2b9809676a34/12885_2023_11412_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/4942e83f0d6e/12885_2023_11412_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/c132452935a0/12885_2023_11412_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/0ef5cb6f3117/12885_2023_11412_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/5a031e9f0f7a/12885_2023_11412_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/855fec73ed44/12885_2023_11412_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/ba813ffcb371/12885_2023_11412_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/7ea5ef6825c6/12885_2023_11412_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/2b9809676a34/12885_2023_11412_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/4942e83f0d6e/12885_2023_11412_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/c132452935a0/12885_2023_11412_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/10536779/0ef5cb6f3117/12885_2023_11412_Fig8_HTML.jpg

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本文引用的文献

1
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Hepatol Int. 2023 Feb;17(1):63-76. doi: 10.1007/s12072-022-10445-1. Epub 2022 Nov 22.
2
Characteristic of Perineural Invasion in Hilar Cholangiocarcinoma Based on Whole-Mount Histologic Large Sections of Liver.基于肝脏全层组织学大切片的肝门部胆管癌神经侵犯特征
Front Oncol. 2022 Mar 8;12:855615. doi: 10.3389/fonc.2022.855615. eCollection 2022.
3
Tyrosine Kinase ROR1 as a Target for Anti-Cancer Therapies.
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Front Oncol. 2021 May 28;11:680834. doi: 10.3389/fonc.2021.680834. eCollection 2021.
4
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Eur Rev Med Pharmacol Sci. 2021 Feb;25(4):1764. doi: 10.26355/eurrev_202102_25051.
5
ROR1-AS1 knockdown inhibits growth and invasion and promotes apoptosis in NSCLC cells by suppression of the PI3K/Akt/mTOR pathway.ROR1-AS1 knockdown 通过抑制 PI3K/Akt/mTOR 通路抑制非小细胞肺癌细胞的生长、侵袭并促进其凋亡。
J Biochem Mol Toxicol. 2021 May;35(5):e22726. doi: 10.1002/jbt.22726. Epub 2021 Jan 24.
6
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7
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