miR-20a 通过调控 PDCD4 作为 lncRNA PTENPL 的 ceRNA 促进膀胱癌细胞的增殖和迁移。
MiR-20a acted as a ceRNA of lncRNA PTENPL and promoted bladder cancer cell proliferation and migration by regulating PDCD4.
机构信息
Urinary Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Mar;24(6):2955-2964. doi: 10.26355/eurrev_202003_20660.
OBJECTIVE
Bladder cancer is the most frequent tumor of the urinary system. Despite variety of new treatment options, bladder cancer remains a main global medical problem. Our purpose was to explore the potential molecular and therapeutic targets of bladder cancer diagnosis.
PATIENTS AND METHODS
The qRT-PCR was used to assess the expression of miR-20a in tissues and cell lines. Counting Cell Kit-8 (CCK-8) assay was carried out to evaluate cell proliferation. Cell migration was calculated using the transwell assay.
RESULTS
The expression of miR-20a increased and PDCD4 decreased in bladder cancer tissues compared with normal tissues. Overexpression of miR-20a promoted T24 cell proliferation and migration, while miR-20a inhibitor suppressed cell proliferation and migration. MiR-20a targeted PDCD4 to regulate its expression in T24 cells. MiR-20a is inversely related to PDCD4 and PTENPL in bladder cancer tissues. Upregulation of PDCD4 suppressed T24 cell proliferation and migration.
CONCLUSIONS
The PTENP1/miR-20a/PTEN axis was involved in the progression of bladder cancer. Our study investigated the function of miR-20a in bladder cancer and provided new insights into the treatment of bladder cancer.
目的
膀胱癌是泌尿系统最常见的肿瘤。尽管有多种新的治疗选择，但膀胱癌仍然是一个主要的全球医学问题。我们的目的是探讨膀胱癌诊断的潜在分子和治疗靶点。
患者和方法
采用 qRT-PCR 检测组织和细胞系中 miR-20a 的表达。用细胞计数试剂盒-8（CCK-8）测定评估细胞增殖。用 Transwell 测定法计算细胞迁移。
结果
与正常组织相比，膀胱癌组织中 miR-20a 的表达增加，PDCD4 减少。miR-20a 的过表达促进了 T24 细胞的增殖和迁移，而 miR-20a 抑制剂则抑制了细胞的增殖和迁移。miR-20a 在 T24 细胞中靶向 PDCD4 以调节其表达。miR-20a 与膀胱癌组织中的 PDCD4 和 PTENPL 呈负相关。PDCD4 的上调抑制了 T24 细胞的增殖和迁移。
结论
PTENP1/miR-20a/PTEN 轴参与了膀胱癌的进展。本研究探讨了 miR-20a 在膀胱癌中的作用，为膀胱癌的治疗提供了新的思路。
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