Yu Gan, Ou Zheng-Yue, Tao Qi-Ye, Wan Guo-Yue, Lu Zong-Hao, Lang Bin
Department of Urology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Nov 20;37(11):1494-1500. doi: 10.3969/j.issn.1673-4254.2017.11.11.
To explore the molecular mechanism underlying the biological function of lncRNA PTENP1 in bladder cancer.
Expressions of PTENP1, PTEN and miR-17 were examined by quantitative reverse transcriptase PCR (qRT-PCR) in 12 bladder cancer tissues. The expression of PTEN was examined by Western blotting in bladder cancer cell lines T24 and 5637 overexpressing PTENP1. Luciferase reporter assay was performed to confirm the targeting of miR-17 to PTENP1 and PTEN. T24 and 5637 cell lines with stable overexpression of PTENP1 and mir-17 were used to investigate effect of PTNE and miR-17 on the function of PTENP1 in bladder cancer.
The expression of miR-17 was up-regulated and PTENP1 and PTEN were down-regulated in bladder cancer tissues, where a positive correlation was found between PTENP1 and PTEN expressions and a negative correlation between PTENP1 and miR-17 (P<0.05). Overexpression of PTENP1 in bladder cancer cell lines T24 and 5637 obviously enhanced the expression of PTEN protein. miR-17 was found to target both PTENP1 and PTEN and promote the growth of bladder cancer. miR-17 could partially restore the tumor-suppressing activity of PTENP1 in bladder cancer.
By binding with miR-17, lncRNA PTENP1 functions as a PTEN competing endogenous RNA (ceRNA) to suppress the progression of bladder cancer.
探讨长链非编码RNA PTENP1在膀胱癌中生物学功能的分子机制。
采用定量逆转录聚合酶链反应(qRT-PCR)检测12例膀胱癌组织中PTENP1、PTEN和miR-17的表达。采用蛋白质免疫印迹法检测过表达PTENP1的膀胱癌T24和5637细胞系中PTEN的表达。进行荧光素酶报告基因检测以证实miR-17对PTENP1和PTEN的靶向作用。利用稳定过表达PTENP1和mir-17的T24和5637细胞系研究PTNE和miR-17对PTENP1在膀胱癌中功能的影响。
膀胱癌组织中miR-17表达上调,PTENP1和PTEN表达下调,PTENP1与PTEN表达呈正相关,PTENP1与miR-17呈负相关(P<0.05)。在膀胱癌T24和5637细胞系中过表达PTENP1可明显增强PTEN蛋白的表达。发现miR-17可靶向PTENP1和PTEN并促进膀胱癌生长。miR-17可部分恢复PTENP1在膀胱癌中的抑癌活性。
lncRNA PTENP1通过与miR-17结合,作为PTEN竞争性内源性RNA(ceRNA)发挥作用,抑制膀胱癌进展。