精原干细胞培养与肿瘤生育力保存
Spermatogonial Stem Cell Culture in Oncofertility.
机构信息
Department of Obstetrics, Gynecology and Reproductive Sciences, Molecular Genetics and Developmental Biology Graduate Program, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA.
Department of Obstetrics, Gynecology and Reproductive Sciences, Molecular Genetics and Developmental Biology Graduate Program, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA.
出版信息
Urol Clin North Am. 2020 May;47(2):227-244. doi: 10.1016/j.ucl.2020.01.001.
Abstract
Infertility caused by chemotherapy or radiation treatments negatively impacts patient-survivor quality of life. The only fertility preservation option available to prepubertal boys who are not making sperm is cryopreservation of testicular tissues that contain spermatogonial stem cells (SSCs) with potential to produce sperm and/or restore fertility. SSC transplantation to regenerate spermatogenesis in infertile adult survivors of childhood cancers is a mature technology. However, the number of SSCs obtained in a biopsy of a prepubertal testis may be small. Therefore, methods to expand SSC numbers in culture before transplantation are needed. Here we review progress with human SSC culture.
摘要
化疗或放疗引起的不孕会对患者-幸存者的生活质量产生负面影响。对于尚未产生精子的青春期前男孩，唯一可用的生育保存选择是冷冻保存含有精原干细胞（SSC）的睾丸组织，这些 SSC 具有产生精子和/或恢复生育能力的潜力。将 SSC 移植到儿童癌症成年幸存者中以再生精子发生是一项成熟的技术。然而，在青春期前睾丸活检中获得的 SSC 数量可能较少。因此，需要在移植前通过培养来扩增 SSC 数量的方法。本文综述了人类 SSC 培养的进展。
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