Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, PR China; OIE Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, PR China.
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, PR China.
Vet Microbiol. 2020 Apr;243:108620. doi: 10.1016/j.vetmic.2020.108620. Epub 2020 Feb 25.
Infectious bursal disease virus (IBDV), the etiological agent of infectious bursal disease (IBD), is a variable RNA virus of Avibirnavirus. Some artificially attenuated vaccine strains of IBDV can adapt to cell culture of chicken embryo fibroblast (CEF) cell or its immortalized cell line DF1 in vitro while wild-type IBDV cannot. In this study, for the first time, a naturally occurring cell-adapted classic strain (genogroup 1) of IBDV named IBD17JL01 was identified in China. Animal experiments showed that IBD17JL01 could severely damage the central immune organ of infected chickens. Sequence analysis of the full-length genome revealed the peculiar molecular characteristics of IBD17JL01 with a few amino acid substitutions that might be involved in cell-tropism, antigenicity, and virulence of IBDV. Identification of this novel strain is beneficial to our understanding of the complexity of the epidemiology of IBDV. And the expansion of viral cell-tropism might increase the potential risk of the reassortment of different IBDVs including the live vaccines.
传染性腔上囊炎病毒（IBDV）是传染性腔上囊炎（IBD）的病原体，属于双 RNA 病毒科禽双 RNA 病毒属。一些 IBDV 的人工致弱疫苗株可以在鸡胚成纤维细胞（CEF）或其永生化细胞系 DF1 体外适应细胞培养，而野毒株则不能。本研究首次在中国鉴定出一种自然发生的细胞适应经典株（基因群 1）IBDV，命名为 IBD17JL01。动物实验表明，IBD17JL01 可严重破坏感染鸡的中枢免疫器官。全长基因组序列分析揭示了 IBD17JL01 的特殊分子特征，其少数氨基酸取代可能参与 IBDV 的细胞嗜性、抗原性和毒力。该新型毒株的鉴定有助于我们了解 IBDV 流行病学的复杂性。病毒细胞嗜性的扩大可能增加不同 IBDV（包括活疫苗）重组的潜在风险。
