Tijani Ahmad A, Adekomi Damilare A, Fature Adedamola A, Adewole Stephen O, Adetunji Adedeji E
Department of Anatomy, Faculty of Basic Medical Sciences, College of Medicine, Ekiti State University, Ado-Ekiti, Nigeria.
Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Osun State University, Osogbo, Nigeria.
Anat Cell Biol. 2020 Mar;53(1):76-83. doi: 10.5115/acb.19.125. Epub 2020 Mar 12.
Exposure to lead (Pb) has been shown to alter the function of central nervous system and affect cholinergic neurons of the visual cortex in animal models. This study sought to investigate the withdrawal symptoms and oxidative stress on the visual cortex after lead exposure. A total of 20 healthy male Wistar rats were randomly divided into two groups (n=10): group A, control, received 10 ml/kg of distilled water for 30 days orally; group B, lead treated group, received 10 mg/kg of lead nitrate solution for 30 days orally. Group B was divided into two subgroups, group B1 serves as non-recovery while B2 serves as recovery (withdrawal). Five rats from each group were sacrificed under ether anesthesia 24 hours after the last oral administration of lead, while the remaining 5 rats (withdrawal subgroup) were sacrificed 30 days after the last oral administration of lead. The visual cortex was grossed from the brain tissue and processed for histology. Blood/serum samples were obtained and markers of oxidative stress (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPX]), and lipid peroxidation (malondialdehyde [MDA]) were analyzed. Lead-exposed rats display a significant reduction in the SOD, CAT, and GPX level as well as increased in MDA level. However, following a recovery period, a non-significant improvement was seen in the histoarchitecture of the visual cortex.
在动物模型中，已证明接触铅（Pb）会改变中枢神经系统的功能并影响视觉皮层的胆碱能神经元。本研究旨在调查铅暴露后视觉皮层的戒断症状和氧化应激。总共20只健康的雄性Wistar大鼠被随机分为两组（n = 10）：A组为对照组，口服10 ml/kg蒸馏水，持续30天；B组为铅处理组，口服10 mg/kg硝酸铅溶液，持续30天。B组又分为两个亚组，B1组为未恢复组，B2组为恢复（戒断）组。在最后一次口服铅后24小时，每组处死5只大鼠，在乙醚麻醉下进行；其余5只大鼠（戒断亚组）在最后一次口服铅后30天处死。从脑组织中取出视觉皮层并进行组织学处理。采集血液/血清样本，分析氧化应激标志物（超氧化物歧化酶[SOD]、过氧化氢酶[CAT]、谷胱甘肽过氧化物酶[GPX]）和脂质过氧化标志物（丙二醛[MDA]）。铅暴露大鼠的SOD、CAT和GPX水平显著降低，MDA水平升高。然而，经过恢复期后，视觉皮层的组织结构有不显著的改善。
