Technion Integrated Cancer Center (TICC), Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Haifa, Israel.
Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA.
Adv Exp Med Biol. 2020;1221:3-59. doi: 10.1007/978-3-030-34521-1_1.
This review summarizes key developments in the heparanase field obtained 20 years prior to cloning of the HPSE gene and nearly 20 years after its cloning. Of the numerous publications and review articles focusing on heparanase, we have selected those that best reflect the progression in the field as well as those we regard important accomplishments with preference to studies performed by scientists and groups that contributed to this book. Apart from a general 'introduction' and 'concluding remarks', the abstracts of these studies are presented essentially as published along the years. We apologize for not being objective and not being able to include some of the most relevant abstracts and references, due to space limitation. Heparanase research can be divided into two eras. The first, initiated around 1975, dealt with identifying the enzyme, establishing the relevant assay systems and investigating its biological activities and significance in cancer and other pathologies. Studies performed during the first area are briefly introduced in a layman style followed by the relevant abstracts presented chronologically, essentially as appears in PubMed. The second era started in 1999 when the heparanase gene was independently cloned by 4 research groups [1-4]. As expected, cloning of the heparanase gene boosted heparanase research by virtue of the readily available recombinant enzyme, molecular probes, and anti-heparanase antibodies. Studies performed during the second area are briefly introduced followed by selected abstracts of key findings, arranged according to specific topics.
这篇综述总结了在克隆 HPSE 基因之前 20 年和之后近 20 年获得的肝素酶领域的关键进展。在众多关注肝素酶的出版物和综述文章中，我们选择了那些最能反映该领域进展的文章，以及那些我们认为具有重要成就的文章，优先选择对本书做出贡献的科学家和小组的研究。除了一般的“引言”和“结语”外，这些研究的摘要基本上按照发表年份原样呈现。由于篇幅限制，我们无法做到客观，也无法包括一些最相关的摘要和参考文献，对此我们深表歉意。肝素酶研究可以分为两个时代。第一个时代始于 1975 年左右，主要涉及鉴定该酶、建立相关的测定系统以及研究其在癌症和其他病理中的生物学活性和意义。简要介绍了该领域的研究，以通俗易懂的方式介绍，随后按时间顺序呈现相关摘要，基本上与 PubMed 中的呈现方式一致。第二个时代始于 1999 年，当时 4 个研究小组[1-4]独立克隆了肝素酶基因。正如预期的那样，肝素酶基因的克隆通过易于获得的重组酶、分子探针和抗肝素酶抗体，极大地推动了肝素酶研究。简要介绍了该领域的研究，随后根据特定主题选择了关键发现的摘要。
