College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang, Liaoning 110036, People's Republic of China.
College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang, Liaoning 110036, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jul 5;235:118331. doi: 10.1016/j.saa.2020.118331. Epub 2020 Apr 1.
A novel hydroxy functionalized naphthalimide-based fluorescent probe (denoted SP2)was successfully designed and synthesized for monitoring of HS in living cells and environmental. In particular, SP2 can detect the HS without assistance of organic solvent or surfactant. When HS is present, the azide group in SP2 was reduced to amine group, resulting in a turn-on fluorescence signal. This remarkable properties of SP2 enable its applications in monitoring ex/endogenous HS in HepG-2 cells and hydrogen sulfide release in laboratories or chemical plants through visual recognition by optical color change. The probe displays highly selective and sensitive recognition to HS, with a low detection limit of 50.8 nM. Futhermore, this work presents the possibility of using naphthalimide-based "logic gate" platform for monitoring HS in biological and environmental samples.
一种新型的羟基功能化萘酰亚胺基荧光探针（命名为 SP2）被成功设计和合成，用于监测活细胞和环境中的 HS。特别是，SP2 可以在没有有机溶剂或表面活性剂辅助的情况下检测 HS。当 HS 存在时，SP2 中的叠氮基团被还原为胺基，导致荧光信号开启。SP2 的这一显著特性使其能够通过光学颜色变化进行目视识别，应用于监测 HepG-2 细胞中外源性/内源性 HS 和实验室或化工厂中硫化氢的释放。该探针对 HS 具有高选择性和灵敏性识别，检测限低至 50.8 nM。此外，这项工作为使用基于萘酰亚胺的“逻辑门”平台监测生物和环境样品中的 HS 提供了可能性。
