School of Medicine, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
Molecules. 2024 Jan 24;29(3):577. doi: 10.3390/molecules29030577.
Hydrogen sulfide (HS) is a significant actor in the virulence and pathogenicity of fungi. The analysis of endogenous HS in fungi benefits the prevention and treatment of pathogenic infections. Herein, a HS-activated turn-on fluorescent probe named was developed for the sensitive and selective detection of HS. Using , the ability of several oral fungi strains to produce HS was identified, which was also validated using a typical chromogenic medium. In addition, was successfully used for the visual sensing of endogenous HS in fungal cells via microscope, flow cytometry, and colony imaging, along with a specific validation with the co-incubation of HS production inhibitors in living cells. Above all, could be used for HS detection, the fluorescent imaging of fungi, and even the identification of related fungi.
硫化氢 (HS) 是真菌毒力和致病性的重要因素。分析真菌内源性 HS 有助于预防和治疗致病感染。在此，开发了一种 HS 激活的荧光探针 ，用于 HS 的灵敏和选择性检测。利用 ，鉴定了几种口腔真菌菌株产生 HS 的能力，这也通过典型的显色培养基得到了验证。此外， 还成功地用于通过显微镜、流式细胞术和菌落成像对真菌细胞内源性 HS 的可视化检测，并在活细胞中与 HS 产生抑制剂共孵育进行了特异性验证。总之， 可用于 HS 检测、真菌的荧光成像，甚至相关真菌的鉴定。
