A Turn-On Fluorescent Probe for Highly Selective Detection and Visualization of Hydrogen Sulfide in Fungi.

Hydrogen sulfide (HS) is a significant actor in the virulence and pathogenicity of fungi. The analysis of endogenous HS in fungi benefits the prevention and treatment of pathogenic infections. Herein, a HS-activated turn-on fluorescent probe named was developed for the sensitive and selective detection of HS. Using , the ability of several oral fungi strains to produce HS was identified, which was also validated using a typical chromogenic medium. In addition, was successfully used for the visual sensing of endogenous HS in fungal cells via microscope, flow cytometry, and colony imaging, along with a specific validation with the co-incubation of HS production inhibitors in living cells. Above all, could be used for HS detection, the fluorescent imaging of fungi, and even the identification of related fungi.