弹道式 DNA 递送及其在高粱中的应用。

Biolistic DNA Delivery and Its Applications in Sorghum bicolor.

机构信息

Centre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Australia.

出版信息

Methods Mol Biol. 2020;2124:197-215. doi: 10.1007/978-1-0716-0356-7_10.

DOI:10.1007/978-1-0716-0356-7_10

PMID:32277455

Abstract

Biolistic DNA delivery has been considered a universal tool for genetic manipulation to transfer exotic genes to cells or tissues due to its simplicity, versatility, and high efficiency. It has been a preferred method for investigating plant gene function in most monocot crops. The first transgenic sorghum plants were successfully regenerated through biolistic DNA delivery in 1993, with a relatively low transformation efficiency of 0.3%. Since then, tremendous progress has been made in recent years where the highest transformation efficiency was reported at 46.6%. Overall, the successful biolistic DNA delivery system is credited to three fundamental cornerstones: robust tissue culture system, effective gene expression in sorghum, and optimal parameters of DNA delivery. In this chapter, the history, application, and current development of biolistic DNA delivery in sorghum are reviewed, and the prospect of sorghum genetic engineering is discussed.

摘要

弹道式 DNA 递送因其简单、多功能和高效率，被认为是将外源基因转移到细胞或组织中的通用遗传操作工具。它已成为研究大多数单子叶作物中植物基因功能的首选方法。1993 年，通过弹道式 DNA 递送首次成功再生了转基因高粱植株，转化效率相对较低，为 0.3%。此后，近年来取得了巨大进展，最高转化效率报道为 46.6%。总的来说，成功的弹道式 DNA 递送系统得益于三个基本基石：强大的组织培养系统、高粱中有效的基因表达和最佳的 DNA 递送参数。本章回顾了弹道式 DNA 递送到高粱中的历史、应用和当前发展，并讨论了高粱遗传工程的前景。