一种高效的含单宁高粱转化方法。

An efficient transformation method for tannin-containing sorghum.

机构信息

College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China.

Research Center of Biology and Agriculture, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.

出版信息

PeerJ. 2023 Mar 14;11:e15066. doi: 10.7717/peerj.15066. eCollection 2023.

DOI:10.7717/peerj.15066

PMID:36935918

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10022505/

Abstract

BACKGROUND

Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing.

METHODS

In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfully exploited using immature embryos (IEs) of Hongyingzi as explants.

RESULTS

Hongyingzi presented two types of calli. Type II calli were found to be the most suitable and effective explants for transformation. After optimization of the geneticin (G418) concentration and tissue culture medium, an average transformation frequency of 27% was achieved. Molecular analyzis showed that all transgenic plants were positive and showed transgenes expression. The inheritance analyzis confirmed that the transgenes could be inherited into the next generation. Thus, we successfully established an efficient transformation system for tannin-containing sorghum and demonstrated the possibility of breaking the restriction imposed by tannins in plants.

摘要

背景

单宁是限制植物转化效率的主要瓶颈。红缨子是一种用于酿造的特殊含单宁高粱品种。

方法

本研究以红缨子的未成熟胚（IEs）为外植体，成功开发了一种高效的含单宁高粱微弹丸转化系统。

结果

红缨子呈现出两种类型的愈伤组织。发现 II 型愈伤组织是最适合和有效的转化外植体。通过优化遗传霉素（G418）浓度和组织培养基，实现了平均 27%的转化频率。分子分析表明，所有转基因植株均为阳性，并表现出转基因表达。遗传分析证实，转基因可以遗传到下一代。因此，我们成功地建立了一个高效的含单宁高粱转化系统，并证明了打破植物中单宁限制的可能性。