Montes G S, Luque E H
Laboratory for Cell Biology and Pathology, University of São Paulo School of Medicine, Brazil.
Acta Anat (Basel). 1988;133(3):192-9. doi: 10.1159/000146639.
A correspondence between the appearance of vaginal smears and the layers of the epithelium from which the cells had desquamated was established in untreated rats during the estrous cycle, in control ovariectomized rats and in spayed rats injected with either estrogen or progesterone. The technique for preparing and staining the smears (modified Shorr's staining procedure) is outlined. A simplified system of classification which allows the accurate identification of the various stages of the reproductive state in the rat is described. Standing estrus, as well as the influence of estrogen on spayed rats, is characterized by marked cornification of the cells and the disappearance of leukocytes. At the end of estrus, the cornified layer is sloughed off and invasion by leukocytes occurs. During diestrus, as well as in untreated ovariectomized rats, the vaginal contents consistently lack cornified cells whereas leukocytes are very plentiful. Proestrus follows diestrus: the vaginal smear is devoid of leukocytes and characterized by nucleated epithelial cells. Pregnancy, as well as the influence of progesterone on ovariectomized rats, is also characterized by epithelial growth and desquamation but at different rates, resulting in the presence of intermediate cells, and polymorphs and mucus forming a noticeable background to the smear. Since vaginal smears display cell pictures characteristic for each hormone after administration of estrogen or progesterone, exfoliate cytology is a good indicator of the stage of the reproductive state in the rat.
在未处理的处于发情周期的大鼠、对照去卵巢大鼠以及注射了雌激素或孕酮的切除卵巢大鼠中，建立了阴道涂片外观与细胞脱落的上皮层之间的对应关系。概述了涂片制备和染色技术（改良的肖尔染色程序）。描述了一种简化的分类系统，该系统能够准确识别大鼠生殖状态的各个阶段。静立发情期以及雌激素对切除卵巢大鼠的影响，其特征是细胞明显角质化且白细胞消失。发情期末期，角质化层脱落，白细胞侵入。在间情期以及未处理的去卵巢大鼠中，阴道内容物始终缺乏角质化细胞，而白细胞非常丰富。发情前期紧接着间情期：阴道涂片无白细胞，以有核上皮细胞为特征。妊娠以及孕酮对去卵巢大鼠的影响，其特征也是上皮生长和脱落，但速率不同，导致出现中间细胞，多形核细胞和黏液在涂片上形成明显的背景。由于在给予雌激素或孕酮后，阴道涂片显示出每种激素特有的细胞图像特征，因此脱落细胞学是大鼠生殖状态阶段的良好指标。
