Igarashi Michihiro, Kawasaki Asami, Ishikawa Yuya, Honda Atsuko, Okada Masayasu, Okuda Shujiro
Departments of Neurochemistry and Molecular Cell Biology, School and Medicine and Graduate School of Medical/Dental Sciences, Japan.
Departments of Neurochemistry and Molecular Cell Biology, School and Medicine and Graduate School of Medical/Dental Sciences, Japan.
J Neurosci Methods. 2020 Jun 1;339:108723. doi: 10.1016/j.jneumeth.2020.108723. Epub 2020 Apr 8.
Phosphorylation is the most important post-translational modification of proteins in many cells, including neurons. Phosphoproteomics is a relatively new technique for comprehensively identifying phosphorylation sites in the whole proteome of a given system. We applied this method to developmental neurobiology research to understand the signaling pathways that regulate the mammalian growth cone, which is formed at the tips of developing neurites to ensure accurate neuronal network formation. Using this powerful technique, we identified at least four phosphorylation sites tightly associated with axon growth. Because phosphoproteomic results include relatively large numbers of phosphopeptides, the data are typically analyzed using bioinformatics. We utilized three bioinformatics tools to identify the responsible protein kinases, the putative functions of the phosphorylated protein groups, and the evolutional aspects of the phosphorylated proteins. Collectively, these data indicate phosphoproteomics is a cutting-edge tool for neuroscience research.
磷酸化是包括神经元在内的许多细胞中蛋白质最重要的翻译后修饰。磷酸化蛋白质组学是一种相对较新的技术，用于全面鉴定给定系统全蛋白质组中的磷酸化位点。我们将此方法应用于发育神经生物学研究，以了解调节哺乳动物生长锥的信号通路，生长锥形成于发育中的神经突尖端，以确保准确的神经网络形成。使用这种强大的技术，我们鉴定出至少四个与轴突生长紧密相关的磷酸化位点。由于磷酸化蛋白质组学结果包含相对大量的磷酸肽，通常使用生物信息学对数据进行分析。我们利用三种生物信息学工具来鉴定相关的蛋白激酶、磷酸化蛋白组的推定功能以及磷酸化蛋白的进化方面。总体而言，这些数据表明磷酸化蛋白质组学是神经科学研究的前沿工具。
