Hansson H A, Helander H F, Holmgren A, Rozell B
Department of Histology, University of Göteborg, Sweden.
Acta Physiol Scand. 1988 Mar;132(3):313-20. doi: 10.1111/j.1748-1716.1988.tb08334.x.
Low levels of thioredoxin and thioredoxin reductase immunoreactivity were demonstrated immunohistochemically in rat gastric epithelial cells. The intensity was influenced by feeding and fasting, the former resulting in diminished reactions. Acute vagotomy, which abolishes basal acid secretion, resulted in a strongly increased thioredoxin immunoreactivity in all gastric epithelial cells. Stimulation of vagotomized rats with pentagastrin and carbachol reduced the levels of thioredoxin and thioredoxin reductase. Atropine and omeprazole (in stimulated, vagotomized rats) completely inhibited acid secretion, but caused different effects on the thioredoxin levels of gastric cells. Atropine restored the thioredoxin immunoreactivity in most gastric epithelial cells to that of the unstimulated, vagotomized controls. Omeprazole, however, did not reverse the effects of stimulation, and, except in the parietal cells, weaker fluorescence was observed. Similar reaction patterns were seen for thioredoxin reductase, although at lower staining intensities. The results demonstrate that thioredoxin and thioredoxin reductase are expressed in resting cells, and to a lower extent in cells with ongoing secretion.
免疫组织化学方法显示，大鼠胃上皮细胞中硫氧还蛋白和硫氧还蛋白还原酶的免疫反应性较低。其强度受进食和禁食的影响，进食会导致反应减弱。急性迷走神经切断术可消除基础胃酸分泌，结果导致所有胃上皮细胞中硫氧还蛋白的免疫反应性大幅增加。用五肽胃泌素和卡巴胆碱刺激迷走神经切断的大鼠会降低硫氧还蛋白和硫氧还蛋白还原酶的水平。阿托品和奥美拉唑（在受刺激的迷走神经切断大鼠中）完全抑制胃酸分泌，但对胃细胞中硫氧还蛋白水平产生不同影响。阿托品使大多数胃上皮细胞中的硫氧还蛋白免疫反应性恢复到未受刺激的迷走神经切断对照水平。然而，奥美拉唑并未逆转刺激的影响，除壁细胞外，观察到较弱的荧光。硫氧还蛋白还原酶也呈现类似的反应模式，尽管染色强度较低。结果表明，硫氧还蛋白和硫氧还蛋白还原酶在静息细胞中表达，而在正在分泌的细胞中表达程度较低。
