Helander H F, Sundell G W
Gastroenterology. 1984 Nov;87(5):1064-71.
In acutely vagotomized rats, gastric acid secretion was stimulated with a combination of carbachol and pentagastrin, and/or inhibited with picoprazole, cimetidine, or l-hyoscyamine. The animals were killed 1 or 3 h later. Using stereologic electron microscopic methods, the relative area of the secretory surface in the parietal cells and the mean size of these cells were estimated. The parietal cells in the superficial quarter of the oxyntic mucosa were larger than those at deeper levels of the mucosa. Moreover, the secretory surface was proportionally larger in the superficial cells than in the deep cells. Stimulation by carbachol and pentagastrin produced an increase in the secretory surface area. Inhibition of stimulated acid secretion by l-hyoscyamine reduced the secretory surface to the level of the unstimulated controls. Cimetidine, given at doses that inhibited stimulated acid secretion, did not alter the mean size of the secretory membrane. After inhibition by picoprazole, stimulated acid secretion was abolished, but the secretory membrane became significantly larger than after cimetidine inhibition. These divergent patterns of morphologic reactions probably reflect the different mechanisms of inhibition at the cellular level.
在急性迷走神经切断的大鼠中，用卡巴胆碱和五肽胃泌素联合刺激胃酸分泌，和/或用匹可拉唑、西咪替丁或l -莨菪碱抑制胃酸分泌。1或3小时后处死动物。采用体视学电子显微镜方法，估计壁细胞分泌表面的相对面积和这些细胞的平均大小。胃底黏膜表层四分之一的壁细胞比黏膜深层的壁细胞大。此外，表层细胞的分泌表面相对比例比深层细胞大。卡巴胆碱和五肽胃泌素刺激导致分泌表面积增加。l -莨菪碱抑制刺激的胃酸分泌使分泌表面减少到未刺激对照组的水平。给予抑制刺激胃酸分泌剂量的西咪替丁，并未改变分泌膜的平均大小。匹可拉唑抑制后，刺激的胃酸分泌被消除，但分泌膜变得比西咪替丁抑制后明显更大。这些不同的形态学反应模式可能反映了细胞水平上不同的抑制机制。
