Hansson H A, Holmgren A, Rozell B, Täljedal I B
Cell Tissue Res. 1986;245(1):189-95. doi: 10.1007/BF00218100.
Thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) have been proposed to be involved in several thiol-dependent reduction-oxidation reactions in cells. Both proteins have been immunohistochemically demonstrated in the periphery of the cytoplasm and in cytoplasmic granules of acinar and islet cells in mouse pancreas. In animals fed ad libitum, the staining for thioredoxin was more intense in the exocrine acinar cells than in the islet cells, whereas that for thioredoxin reductase was more intense in the endocrine than in the exocrine pancreas. In the islets of fed mice all endocrine cell types showed about the same staining intensity for thioredoxin, while thioredoxin reductase was greatly enriched in the somatostatin-containing D cells. Starvation overnight caused an increased staining for both proteins in the acinar cells as well as in the islets. Under conditions of starvation, thioredoxin reductase, in contrast to thioredoxin, appeared to increase preferentially in the islet B cells, as compared with the D cells. Cysteamine treatment reduced the staining for somatostatin and for thioredoxin reductase in the D cells without any obvious effect on the other pancreatic cells. The results are compatible with a role for thioredoxin and thioredoxin reductase in secretion.
硫氧还蛋白和硫氧还蛋白还原酶（NADPH - 氧化型硫氧还蛋白氧化还原酶，E.C. 1.6.4.5）被认为参与细胞内多种依赖硫醇的氧化还原反应。这两种蛋白质已通过免疫组织化学方法在小鼠胰腺腺泡细胞和胰岛细胞的细胞质周边及细胞质颗粒中得到证实。在随意进食的动物中，硫氧还蛋白在外分泌腺泡细胞中的染色比在胰岛细胞中更强烈，而硫氧还蛋白还原酶在内分泌胰腺中的染色比在外分泌胰腺中更强烈。在进食小鼠的胰岛中，所有内分泌细胞类型对硫氧还蛋白的染色强度大致相同，而硫氧还蛋白还原酶在含生长抑素的D细胞中大量富集。禁食过夜导致腺泡细胞和胰岛中这两种蛋白质的染色增加。在饥饿条件下，与硫氧还蛋白相比，硫氧还蛋白还原酶在胰岛B细胞中似乎比在D细胞中优先增加。半胱胺处理降低了D细胞中生长抑素和硫氧还蛋白还原酶的染色，而对其他胰腺细胞没有明显影响。这些结果与硫氧还蛋白和硫氧还蛋白还原酶在分泌过程中的作用相符。
