The Sixth People's Hospital of Zhengzhou, Zhengzhou, 450000, China.
Arch Virol. 2020 Jun;165(6):1453-1461. doi: 10.1007/s00705-020-04606-6. Epub 2020 Apr 11.
Human immunodeficiency virus type 1 (HIV-1) infection remains a severe public health problem worldwide. In this study, we investigated the distribution of HIV-1 subtypes and the prevalence of drug resistance mutations (DRMs) among patients with HIV-1 infection in Henan Province, China. HIV-1 strains in blood samples taken from inpatients and outpatients visiting the Sixth People's Hospital of Zhengzhou from August 2017 to July 2019 with a viral load (VL) greater than 1000 copies/ml were subjected to subtype and DRMs analysis. Out of a total of 769 samples, subtype and DRM data were obtained from 657 (85.43%) samples. Phylogenetic analysis based on partial pol gene sequences indicated that the most commonly found genotype was subtype B (45.51%, 299/657), followed by CRF01_AE (28.61%, 188/657), CRF07_BC (15.68%, 103/657), CRF08_BC (0.76%, 5/657), C (0.61%, 4/657), A (0.30%, 2/657), and others (8.52%, 56/657). Circulating recombinant forms (CRFs) were most commonly found in patients who were naïve to antiretroviral treatment (ART) (68.67%, 160/233). The percentage of patients with one or more major drug-resistance mutations was 50.99% (335/657), and it was 6.44% (15/233) in ART-naive patients that were primarily infected with subtype B (17.74%). Resistance mutations were most common at codons 65, 103, 106, 184, and 190 of the reverse transcriptase gene and codon 46 of the protease gene. Our study provides detailed information about the distribution of HIV-1 subtypes and the incidence of drug resistance mutations of different subtypes in ART-experienced and naïve patients. This can guide policymakers in making decisions about treatment strategies against HIV-1.
人类免疫缺陷病毒 1 型（HIV-1）感染仍然是全球严重的公共卫生问题。本研究调查了中国河南省 HIV-1 感染患者中 HIV-1 亚型的分布和耐药突变（DRM）的流行情况。对 2017 年 8 月至 2019 年 7 月期间在郑州市第六人民医院就诊的病毒载量（VL）大于 1000 拷贝/ml 的住院和门诊患者的血液样本中的 HIV-1 株进行了亚型和 DRM 分析。在总共 769 个样本中，从 657 个（85.43%）样本中获得了亚型和 DRM 数据。基于部分 pol 基因序列的系统发育分析表明，最常见的基因型是亚型 B（45.51%，299/657），其次是 CRF01_AE（28.61%，188/657），CRF07_BC（15.68%，103/657），CRF08_BC（0.76%，5/657），C（0.61%，4/657），A（0.30%，2/657）和其他（8.52%，56/657）。在未接受抗逆转录病毒治疗（ART）的患者中，循环重组形式（CRFs）最为常见（68.67%，160/233）。有一个或多个主要耐药突变的患者比例为 50.99%（335/657），在主要感染亚型 B（17.74%）的未接受 ART 治疗的患者中为 6.44%（15/233）。耐药突变最常见于逆转录酶基因的 65、103、106、184 和 190 密码子以及蛋白酶基因的 46 密码子。本研究提供了关于 ART 经验丰富和未经验患者中 HIV-1 亚型分布和不同亚型耐药突变发生率的详细信息。这可以为决策者制定针对 HIV-1 的治疗策略提供指导。
