College of Life Science, Northeast Agricultural University, Harbin, 150030, China.
Key Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science, Guiyang, 550083, China.
Mol Biol Rep. 2020 May;47(5):3475-3484. doi: 10.1007/s11033-020-05433-3. Epub 2020 Apr 11.
Small ubiquitin-like modifier (SUMO) participates in post-translational modification of various target proteins. SUMOylation is an important molecular regulatory mechanism for plants to respond to abiotic stress. In the present study, GmSUMO2 gene was isolated from soybean seedlings for further study because of the highest expression level among these six SUMO genes in soybean. qRT-PCR results showed that GmSUMO2 gene were detected in root, leaf, cotyledon, seed root, flower, pod and seed, with the highest transcription level in cotyledon. Moreover, GmSUMO2 gene was transcriptionally regulated by 200 mM NaCl, 42 °C, 25 μM abscisic acid (ABA) and 20% PEG6000 during the 24 h period of treatment. Besides, western blot analysis using AtSUMO1 antibody indicated that the free SUMO levels and SUMOylation dynamics were regulated by ABA stimulus. Functional analysis indicated that overexpression of GmSUMO2 gene in soybean hairy roots accentuated the sensitivity to exogenous ABA. Furthermore, the expression levels of ABI3, ABI5, SnRK1.1 and SnRK1.2 were differentially regulated by GmSUMO2 in transgenic soybean hairy roots. Overall, these results provided a preliminary understanding of molecular characterization, expression and function of GmSUMO2 in soybean.
小泛素样修饰物 (SUMO) 参与各种靶蛋白的翻译后修饰。SUMOylation 是植物应对非生物胁迫的重要分子调控机制。在本研究中，由于这六个 SUMO 基因在大豆中的表达水平最高，因此从大豆幼苗中分离出 GmSUMO2 基因进行进一步研究。qRT-PCR 结果表明，GmSUMO2 基因存在于根、叶、子叶、种根、花、荚和种子中，在子叶中转录水平最高。此外，GmSUMO2 基因在处理 24 小时期间受到 200 mM NaCl、42°C、25 μM 脱落酸 (ABA) 和 20%PEG6000 的转录调控。此外，使用 AtSUMO1 抗体进行的 Western blot 分析表明，游离 SUMO 水平和 SUMOylation 动态受 ABA 刺激调节。功能分析表明，大豆毛状根中超表达 GmSUMO2 基因会加重对外源 ABA 的敏感性。此外，在转基因大豆毛状根中，GmSUMO2 差异调节 ABI3、ABI5、SnRK1.1 和 SnRK1.2 的表达水平。总的来说，这些结果初步了解了 GmSUMO2 在大豆中的分子特征、表达和功能。
