Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China.
Anhui Science and Technology University, Fengyang 233100, China.
Int J Mol Sci. 2020 Mar 21;21(6):2177. doi: 10.3390/ijms21062177.
Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes () have been reported to exist in the soybean () genome. In spite of this, little information is currently available regarding In this study, were cloned and characterized. genes encode proteins possessing transcriptional activation activity in yeast cells. , , and gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, showed a significantly induced expression in response to all stress treatments. Overexpression of in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress.
植物单锌指（VOZ）转录因子是一种植物特异性的单锌指型转录激活因子，参与调节许多生物学过程，如花诱导和发育、抵御病原体和对多种非生物胁迫的响应。已有报道称，大豆基因组中存在 6 个 VOZ 转录因子编码基因（）。尽管如此，目前关于 的信息还很少。在本研究中，克隆并表征了 。这些基因编码的蛋白在酵母细胞中具有转录激活活性。、、和 基因产物广泛分布在细胞质中，而 GmVOZ1G 主要位于细胞核中。在脱水、盐和水杨酸（SA）胁迫条件下， 表现出不同的表达谱。其中， 对所有胁迫处理均表现出显著诱导表达。在大豆毛状根中过表达 导致对干旱和盐胁迫的耐受性增加。相反，抑制 表达的 RNAi 大豆毛状根对这两种胁迫更为敏感。在干旱处理下，过表达毛状根的大豆复合植株具有更高的相对水含量（RWC）。在干旱和盐胁迫下，过表达毛状根的大豆复合幼苗中丙二醛（MDA）积累减少，而过氧化物酶（POD）和超氧化物歧化酶（SOD）活性增加。在 RNAi 系中，每个生理参数都得到了相反的结果。总之， 正向调节大豆毛状根对干旱和盐胁迫的耐受性。我们的研究结果将有助于在非生物胁迫下对大豆 VOZ 转录因子的功能进行表征。
