Department of Chemistry, Faculty of Science, Tanta University, Egypt.
Department of Chemistry, Faculty of Science, Tanta University, Egypt.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110773. doi: 10.1016/j.msec.2020.110773. Epub 2020 Feb 26.
Herein, a 3D hierarchical blossom-like of Montmorillonite-ZnO (MMt/ZnO) micro-hybrids modified sensors have been successfully fabricated as an extraordinarily electrochemical sensor for detecting of the Diltiazem hydrochloride (DZM.HCl). The 3D hierarchical blossom-like of ZnO and series of MMt/ZnO hybrids have been synthesized using different contents of MMt [FMZ] via a hydrogel polymer template method using alginate ions. The effect of incorporation of different contents of MMt on the morphology, surface area of hybrids were investigated using Fourier transform infrared (FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM), Energy-dispersive X-ray spectroscopy (EDS), Brunauer-Emmett-Teller (BET) surface area method, and High-resolution transmission electron microscopy (HR-TEM). The obtained hybrid [FMZ] with 2.0% of MMt presented the most perfect blossom-like morphology and the highest surface area (190.06 m/g) with the lowest resistivity. The hierarchical structure of [FMZ] reveals nanospheres of ZnO with an average diameter of 5.49 nm, which are assembled into nanorods followed by assembling to form a blossom-like shape with the inclusion of MMt peeled layers inside the rod with d-spacing ranges from 1.1-7.4 nm. Meanwhile, the implemented modified sensor 1.0% [FMZ] CPS retained excellent conductivity and electrocatalytic activity as appraised from the cyclic voltammetry (CV) measurements. Consequently, the electrochemical behavior and the oxidation mechanism of DZM.HCl drug has been investigated at the surface of the constructed sensor. Under the optimum operational conditions, the proposed sensor was successfully achieved detection limits 0.177, and 0.21 nmol·L of DZM.HCl in a commercial and human biological fluid (Serum samples), respectively. The constructed sensor accomplished an appropriate accuracy and free of obstruction from other ordinarily drug excipients.
本文成功制备了一种 3D 分层花状蒙脱土-氧化锌（MMt/ZnO）微混合修饰传感器，作为一种用于检测盐酸地尔硫卓（DZM.HCl）的电化学传感器。通过水凝胶聚合物模板法，使用海藻酸钠离子，以不同含量的 MMt [FMZ] 合成了 3D 分层花状 ZnO 和一系列 MMt/ZnO 混合物。通过傅里叶变换红外（FTIR）、X 射线衍射（XRD）、场发射扫描电子显微镜（FE-SEM）、能谱（EDS）、BET 比表面积法和高分辨率透射电子显微镜（HR-TEM）研究了不同含量的 MMt 对混合物形貌、比表面积的影响。所得的混合[FMZ]中含有 2.0%的 MMt，呈现出最完美的花状形态和最高的比表面积（190.06 m/g），电阻最低。[FMZ]的分层结构揭示了 ZnO 的纳米球，其平均直径为 5.49nm，这些纳米球组装成纳米棒，然后组装成花状，在棒内包含 MMt 剥落层，层间距范围为 1.1-7.4nm。同时，实施的改性传感器 1.0%[FMZ] CPS 保留了优异的导电性和电催化活性，这可以从循环伏安（CV）测量中得到评估。因此，研究了在构建的传感器表面上 DZM.HCl 药物的电化学行为和氧化机制。在最佳操作条件下，该传感器成功实现了在商业和人体生物流体（血清样品）中对 DZM.HCl 的检测限分别为 0.177 和 0.21 nmol·L。构建的传感器实现了适当的准确性，并且不受其他通常药物赋形剂的干扰。
