Department of Nano-Bioengineering, College of Life Science and Bioengineering, Incheon National University, Republic of Korea.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110795. doi: 10.1016/j.msec.2020.110795. Epub 2020 Mar 2.
In order to develop a rapid and high fidelity process for DNA self-assembly with patterning, the pattern of thymine dimerization is presented onto electrostatically bound DNA substrate by photolithography. The ability of binding for the process, which is attenuated conditions such as contact with photomask and washing by solution buffer is evaluated by X-ray photoelectron spectroscopy (XPS). Through thymine dimerization and hybridization chain reaction (HCR), DNA patterns, including multi-patterns, are demonstrated. For expansion to protein molecular patterning, the target DNA is tethered to biotin, allowing patterning with streptavidin linked fluorophores such as Cy3-streptavidin and phyecocayine-streptavidin.
为了开发具有图案化的快速和高保真度的 DNA 自组装方法，通过光刻将胸腺嘧啶二聚体的图案呈现到静电结合的 DNA 基底上。通过 X 射线光电子能谱（XPS）评估了该过程的结合能力，该过程在与光刻掩模接触和用溶液缓冲液洗涤等减弱条件下会减弱。通过胸腺嘧啶二聚化和杂交链式反应（HCR），展示了包括多图案在内的 DNA 图案。为了扩展到蛋白质分子图案化，将目标 DNA 连接到生物素上，允许使用与 Cy3-链霉亲和素和 phycoerythrin-链霉亲和素等荧光染料连接的链霉亲和素进行图案化。
