School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; JiaxingYaojiao Medical Device Co. Ltd., 321 Jiachuang Road, Jiaxing 314032, China.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110766. doi: 10.1016/j.msec.2020.110766. Epub 2020 Feb 19.
Conduit scaffolds have potential applications in tissue engineering as nerve conduits, urological stent and blood vessel graft. Zein is a well-reported biopolymer in tissue engineering and drug delivery systems. Herein, we prepared ciprofloxacin loaded zein conduits using a facile rolling method. Zein conduits (ZCs) were evaluated for physical structure, porosity, bending stiffness, degradation, drug release, in vitro and in vivo antibacterial efficacy and cells toxicity. ZCs showed porous structure with porosity > 60 % and good mechanical strength with bending stiffness of 28.54 N.mm. Slow enzymatic degradation (87 % in 30 days) was also observed for ZCs. Slow release of ciprofloxacin up to 42 days was observed that could assure prevention of post-implantation infection. In vitro and in vivo antibacterial study verified the short-time and long-time antibacterial efficacy of zein conduits on Gram-positive and Gram-negative bacteria. Live/dead measurement and CCK-8 assay on L929 cells demonstrated good cell compatibility for all zein conduits (>90 % cell viability and cells proliferation in 3 days). Overall, the rolling method could be exploited for preparation of ciprofloxacin loaded zein conduits, which had the potential for tissue engineering applications.
导管支架在组织工程中有潜在的应用，如神经导管、泌尿科支架和血管移植物。玉米醇溶蛋白是组织工程和药物输送系统中报道较多的生物聚合物。本文采用简便的滚压法制备了载环丙沙星的玉米醇溶蛋白导管。对玉米醇溶蛋白导管（ZCs）的物理结构、孔隙率、弯曲刚度、降解、药物释放、体外和体内抗菌效果和细胞毒性进行了评价。ZCs 表现出多孔结构，孔隙率>60%，具有良好的机械强度，弯曲刚度为 28.54 N.mm。还观察到 ZCs 的酶促降解缓慢（30 天内降解 87%）。观察到环丙沙星的缓慢释放长达 42 天，可确保预防植入后的感染。体外和体内抗菌研究验证了玉米醇溶蛋白导管对革兰氏阳性菌和革兰氏阴性菌的短期和长期抗菌效果。L929 细胞的死活测定和 CCK-8 分析表明，所有玉米醇溶蛋白导管的细胞相容性良好（>90%细胞活力和细胞增殖在 3 天内）。总的来说，滚压法可用于制备载环丙沙星的玉米醇溶蛋白导管，具有组织工程应用的潜力。
