Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, PR China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, PR China.
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, PR China.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110809. doi: 10.1016/j.msec.2020.110809. Epub 2020 Mar 3.
In this study, magnetic graphene oxide (MGO) nanohybrids were firstly fabricated by loading FeO NPs onto GO nanosheets and then novel nisin grafted MGO (nisin-g-MGO) nanohybrids were prepared by a facile method. The nisin-g-MGO nanohybrids exhibit strong enough magnetic separation capacity. The magnetic saturation (Ms) of nisin-g-MGO nanohybrids was 23.3 emu/g that enables nisin-g-MGO nanohybrids can be rapidly and completely separated from the black and stable suspension by an external magnet. The antibacterial studies of nisin-g-MGO nanohybrids show great antibacterial performance against Staphylococcus aureus and Bacillus subtilis, respectively. Moreover, the nisin-g-MGO nanohybrids exhibit long-term antibacterial stability that they still keep their good antibacterial activity after six months storage. Meanwhile, the fabricated nisin-g-MGO nanohybrids exhibit good biocompatibility without any obvious effect on HEK293 cell viability due to the calculated cell viabilities were more than 95%. And no observed changes in cell morphology were found in nisin-g-MGO nanohybrids treated HEK293 cells. Hence, the fabricated nisin-g-MGO nanohybrids exhibit great potentials in antibacterial application.
在这项研究中，首先通过将 FeO NPs 负载到 GO 纳米片上制备了磁性氧化石墨烯（MGO）纳米杂化物，然后通过简便的方法制备了新型乳链菌肽接枝 MGO（nisin-g-MGO）纳米杂化物。nisin-g-MGO 纳米杂化物具有足够强的磁性分离能力。nisin-g-MGO 纳米杂化物的饱和磁化强度（Ms）为 23.3 emu/g，使得 nisin-g-MGO 纳米杂化物可以通过外部磁铁快速且完全地从黑色稳定悬浮液中分离出来。nisin-g-MGO 纳米杂化物的抗菌研究表明，其对金黄色葡萄球菌和枯草芽孢杆菌均具有很强的抗菌性能。此外，nisin-g-MGO 纳米杂化物具有长期的抗菌稳定性，在储存六个月后仍保持良好的抗菌活性。同时，由于计算出的细胞活力超过 95%，所制备的 nisin-g-MGO 纳米杂化物表现出良好的生物相容性，对 HEK293 细胞活力没有任何明显影响。并且在 nisin-g-MGO 纳米杂化物处理的 HEK293 细胞中未发现细胞形态发生变化。因此，所制备的 nisin-g-MGO 纳米杂化物在抗菌应用中具有很大的潜力。
