Ranjbar Kermani Fahimeh, Amini Kafi-Abad Sedigheh, Mousavi Hossein Kamran, Maghsudlu Mahtab, Sharifi Zohreh, Mansournia Mohammad Ali
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Med J Islam Repub Iran. 2019 Dec 24;33:149. doi: 10.34171/mjiri.33.149. eCollection 2019.
Hepatitis C virus (HCV) is a blood born virus and the leading cause of advanced hepatitis disease. HCV genotype 3a is predominant among Iranian blood donors. The aim of this study was to evaluate the relationship between HCV genotype and HCV viral load. In this analytical cross-sectional study 106 anti-HCV positive and HCV RNA positive blood donors referred to Iranian blood centers across the county were entered. HCV viral loads were determined by an in-house one step Taq Man Real-Time RT-PCR assay. Penalized logistic regression was performed for data analysis. STATA software version 13 was used for statistical analysis. The mean age was 37.94 ± 9.04 years ranged from 19 to 58 years. Male gender included 104 (98.1%) of subjects. 31, 10 and 65 subjects were infected with genotypes1a, 1b, and 3a, respectively. The mean viral load was 1.44 × 106 ± 4.5× 105 IU/ml. HCV viral load was not significantly different among subjects infected with HCV genotypes 1, 1.49 × 10 6 ± 4.57 × 10 6 IU/ml compare to genotype 3, 1.40 × 10 6 ± 5.58 4.58 × 106 IU/ml (p=0.93). Although not significant, the frequency of subjects with high viral load (> 800,000 IU/ml) was higher in subjects infected with genotype 3 than those of genotype 1. No associations were found between demographic characteristics and HCV genotype. Although the study was unable to find any association between HCV genotype and HCV viral load/ HCV viral load group, it highlighted the role of high viral load in the high circulation of HCV genotype 3a among Iranian blood donors.
丙型肝炎病毒（HCV）是一种血液传播病毒，也是晚期肝病的主要病因。HCV基因3a型在伊朗献血者中占主导地位。本研究的目的是评估HCV基因型与HCV病毒载量之间的关系。在这项分析性横断面研究中，纳入了106名抗-HCV阳性且HCV RNA阳性的献血者，他们来自全国各地的伊朗血液中心。HCV病毒载量通过内部一步法Taq Man实时逆转录聚合酶链反应（RT-PCR）测定法确定。采用惩罚逻辑回归进行数据分析。使用STATA软件版本13进行统计分析。平均年龄为37.94±9.04岁，范围从19岁至58岁。男性受试者有104名（98.1%）。分别有31、10和65名受试者感染了基因1a型、1b型和3a型。平均病毒载量为1.44×10⁶±4.5×10⁵国际单位/毫升。感染HCV基因1型的受试者的HCV病毒载量为1.49×10⁶±4.57×10⁶国际单位/毫升，与基因3型的1.40×10⁶±5.58×4.58×10⁶国际单位/毫升相比，差异无统计学意义（p = 0.93）。虽然不显著，但病毒载量高（>800,000国际单位/毫升）的受试者在感染基因3型的人群中比基因1型的人群中更为常见。未发现人口统计学特征与HCV基因型之间存在关联。尽管该研究未能发现HCV基因型与HCV病毒载量/HCV病毒载量组之间存在任何关联，但它突出了高病毒载量在HCV基因3a型在伊朗献血者中高传播率中的作用。
