Kaur Devinder, Prabhakar K, Das Subhashis
Sri. Devaraj Urs Medical College, SDUMC, Kolar, Karnataka, India.
Bioinformation. 2022 Apr 30;18(4):387-391. doi: 10.6026/97320630018387. eCollection 2022.
Decisions and disease progression prediction, understanding the distribution of the hepatitis C virus (HCV) genotype and its association with viral load is significant for treatment. Therefore, it is of interest to document the distribution pattern of HCV genotypes and their association with viral load among HCV infected patients in Kolar, Karnataka. Seventy-four HCV-positive patients and not on antiviral therapy were enrolled from R.L. Jalappa hospital in Kolar, Karnataka. Blood samples were taken and demographics were recorded. HCV RNA was isolated after plasma was separated. qPCR was performed to measure the viral load, and RT-PCR was performed to determine the genotype. Genotype 3 was the prevalent (n=11, 40.7%) followed by genotype 4 (n=8, 29.6%), 2 (n=6, 22.2%), 1 (n= 13.7%), and mix (n=1, 13.7%). The median viral load of genotype 3 was a 2,87,835 IU/mL (IQR 10, 780-3, 71, 66) , genotype 2 was 81,030 IU/mL (IQR 66,495-95,565), genotype 4 was 43, 410 IU/mL (IQR 38, 355-48, 465) belongs to viral load less than 8,00,000 IU/mL. The median viral load genotype 3 was a 1, 05, 19, 500 IU/mL (IQR 49, 37, 250-2, 36, 71, 500), genotype 2 was 2,55,99,000 IU/mL (IQR 2,00,10,000-32,725,500), genotype 4 was 1,67,40,000 IU/mL (IQR 1,45,50,000-17,493,000) belonging to viral load more than 8,00,000 IU/mL category. A correlation between genotype and viral load was observed (p =1.5x10-12), of which genotype 3 showed a high viral load. Thus, HCV genotypes 1 2, 3, 4, and mixed genotype was observed in the patients studied. HCV genotype was associated with viral load in patient plasma. This data finds use in the treatment and prevention of hepatitis C in Kolar, Karnataka.
决策与疾病进展预测方面，了解丙型肝炎病毒（HCV）基因型的分布及其与病毒载量的关联对于治疗具有重要意义。因此，记录卡纳塔克邦科拉尔地区HCV感染患者中HCV基因型的分布模式及其与病毒载量的关联很有必要。从卡纳塔克邦科拉尔的R.L.贾拉帕医院招募了74例未接受抗病毒治疗的HCV阳性患者。采集血样并记录人口统计学信息。分离血浆后提取HCV RNA。进行qPCR检测病毒载量，进行RT-PCR确定基因型。基因型3最为常见（n = 11，40.7%），其次是基因型4（n = 8，29.6%）、2（n = 6，22.2%）、1（n = 13.7%）和混合基因型（n = 1，13.7%）。病毒载量低于800,000 IU/mL时，基因型3的中位病毒载量为287,835 IU/mL（IQR 10,780 - 371,66），基因型2为81,030 IU/mL（IQR 66,495 - 95,565），基因型4为43,410 IU/mL（IQR 38,355 - 48,465）。病毒载量高于800,000 IU/mL时，基因型3的中位病毒载量为1,0519,500 IU/mL（IQR 4937,250 - 236,71,500），基因型2为2,5599,000 IU/mL（IQR 2,0010,000 - 32,725,500），基因型4为1,6740,000 IU/mL（IQR 1,4550,000 - 17,493,000）。观察到基因型与病毒载量之间存在相关性（p = 1.5x10 - 12），其中基因型3显示出高病毒载量。因此，在所研究的患者中观察到了HCV基因型1、2、3、4和混合基因型。HCV基因型与患者血浆中的病毒载量相关。该数据可用于卡纳塔克邦科拉尔地区丙型肝炎的治疗和预防。
