Lozins Roberts, Selga Tūrs, Ozoliņš Dzintars
Residency Development Program, University of Latvia, Riga, Latvia.
Faculty of Medicine, University of Latvia, Riga, Latvia.
Heliyon. 2020 Apr 6;6(4):e03678. doi: 10.1016/j.heliyon.2020.e03678. eCollection 2020 Apr.
In this study, spectrophotometry was used to measure changes in the absorbance properties of yeast, Gram-positive, and Gram-negative bacteria after their attachment to silicon dioxide microparticles (silica). The goal of this study was to determine whether spectrophotometry is an effective method to distinguish these microorganisms from one another and determine whether they have an affinity for silicon dioxide. The experiments were performed by examining the light absorption properties of yeast, Gram-positive and Gram-negative bacteria in a spectrophotometer, both with and without silicon dioxide microparticles. The experiments produced a number of promising results. First, the spectrophotometer graphs of yeast were noticeably different from those of both Gram-positive and Gram-negative bacteria. Second, the absorption of light in both Gram-positive and Gram-negative bacteria occurred at near infrared range (700-1500 nm) and, unlike yeast, the wavelengths increased when silicon dioxide microparticles were added to the suspension. When silicon dioxide microparticles were added to yeast, the absorption of light decreased during the entire wavelength interval of the spectrophotometer measurement. These results indicate that bacteria have an affinity for silicon dioxide, and that spectrophotometry may be used to distinguish yeast from bacteria and, possibly, different bacterial types from one another.
在本研究中，使用分光光度法测量酵母、革兰氏阳性菌和革兰氏阴性菌附着于二氧化硅微粒（硅胶）后吸光度特性的变化。本研究的目的是确定分光光度法是否是一种区分这些微生物并确定它们对二氧化硅是否具有亲和力的有效方法。实验通过在分光光度计中检测有无二氧化硅微粒时酵母、革兰氏阳性菌和革兰氏阴性菌的光吸收特性来进行。实验产生了一些有前景的结果。首先，酵母的分光光度计图谱与革兰氏阳性菌和革兰氏阴性菌的图谱明显不同。其次，革兰氏阳性菌和革兰氏阴性菌的光吸收均发生在近红外范围（700 - 1500纳米），与酵母不同的是，向悬浮液中添加二氧化硅微粒时波长会增加。当向酵母中添加二氧化硅微粒时，在分光光度计测量的整个波长区间内光吸收都会降低。这些结果表明细菌对二氧化硅具有亲和力，并且分光光度法可用于区分酵母与细菌，甚至可能用于区分不同类型的细菌。
