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用新型“特洛伊木马”载体——柠檬酸盐纳米颗粒培养细胞

Feeding Cells with a Novel "Trojan" Carrier: Citrate Nanoparticles.

作者信息

Rimsueb Natchanon, Cherdchom Sarocha, Aksornkitti Vitavat, Khotavivattana Tanatorn, Sereemaspun Amornpun, Rojanathanes Rojrit

机构信息

Faculty of Science, Department of Chemistry, Chulalongkorn University, Phayathai Road, Wangmai, Patumwan, Bangkok 10330, Thailand.

Chula Medical Innovation Center (CMIC), Nanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Rama 4 Road, Patumwan, Bangkok 10330, Thailand.

出版信息

ACS Omega. 2020 Mar 27;5(13):7418-7423. doi: 10.1021/acsomega.0c00032. eCollection 2020 Apr 7.

DOI:10.1021/acsomega.0c00032
PMID:32280883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7144169/
Abstract

In this work, the preparation of novel calcium citrate (CaCit) nanoparticles (NPs) has been disclosed and the use of these NPs as "Trojan" carriers has been demonstrated. The concentration ratio between calcium ions and citrate ions was optimized, yielding spherical NPs with size in the range of 100-200 nm. Additionally, a fluorescent dye, fluorescein isothiocyanate (FITC), was successfully encapsulated by the coprecipitation method. The products were characterized by thermogravimetric analysis and scanning electron microscopy. The cellular uptake was investigated by incubating the synthesized fluorescent-tagged NPs with human keratinocytes using a confocal microscope. The accumulation of the FITC in the cells suggested that the CaCit NPs can potentially be used as novel drug carriers.

摘要

在本研究中，已公开了新型柠檬酸钙（CaCit）纳米颗粒（NPs）的制备方法，并证明了这些NPs作为“特洛伊木马”载体的用途。优化了钙离子与柠檬酸根离子之间的浓度比，得到了尺寸在100-200nm范围内的球形NPs。此外，通过共沉淀法成功地包封了一种荧光染料，异硫氰酸荧光素（FITC）。通过热重分析和扫描电子显微镜对产物进行了表征。使用共聚焦显微镜，通过将合成的荧光标记NPs与人角质形成细胞孵育来研究细胞摄取情况。FITC在细胞中的积累表明CaCit NPs有可能用作新型药物载体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/e9b346504ee8/ao0c00032_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/cc8a840b964a/ao0c00032_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/f15acac558a3/ao0c00032_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/d1c424521a37/ao0c00032_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/2ccebc7ddd19/ao0c00032_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/8086687c5316/ao0c00032_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/bf9077957513/ao0c00032_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/d647c6dc12f9/ao0c00032_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/51a0c3f9c7a7/ao0c00032_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/6f8f2e2bb060/ao0c00032_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/e9b346504ee8/ao0c00032_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/cc8a840b964a/ao0c00032_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/f15acac558a3/ao0c00032_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/d1c424521a37/ao0c00032_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/2ccebc7ddd19/ao0c00032_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/8086687c5316/ao0c00032_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/bf9077957513/ao0c00032_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/d647c6dc12f9/ao0c00032_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/51a0c3f9c7a7/ao0c00032_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/6f8f2e2bb060/ao0c00032_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6d6/7144169/e9b346504ee8/ao0c00032_0002.jpg

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