National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommiaulmoides, Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie, 427000, PR China.
Institute of Food Science, Jishou University, Jishou, 416000, PR China.
J Food Sci. 2020 May;85(5):1489-1499. doi: 10.1111/1750-3841.15103. Epub 2020 Apr 13.
Eucommia ulmoides Oliver seed oil (E.u oil) as a functional oil is rich in many natural active components such as α-linolenic acid (56% to 63%), vitamin E, aucubin, and so on. In this study, water-in-oil (W/O) microemulsions composed of Eucommia ulmoides Oliver seed oil, distilled water, a blend of Sorbitan monooleate 80 (Span 80) and Polysorbate (20) sorbitan monooleate (Tween 80), and propylene glycol were prepared for improving the compatibility of Eucommia ulmoides Oliver seed oil. Pseudoternary phase diagrams were built to illustrate the phase behavior of the microemulsions, based on hydrophilic-lipophilic balance values, cosurfactant type, the proportion of cosurfactant, and the changing environmental stress. Dynamic light scattering, transmission electron microscopy, and electrical conductivity measurements were performed to characterize the microstructural aspects. The optimum process conditions at which the Eucommia ulmoides Oliver seed oil-loaded microemulsion had good tolerance to pH and salinity were: Propylene glycol served as cosurfactant, water-Propylene glycol, and Span 80-Tween 80 ratios separately kept constant at 1:1 and 6:4. These microemulsions with narrow size distribution, nanoscale particle size (below 60 nm), transparent appearance had a wide range of oil phase content and free-radical scavenging capacity toward DPPH and ABTS radicals with half-maximal inhibitory concentration (IC ) values of 49.20 and 33.43 mg/mL, respectively. PRACTICAL APPLICATION: This nanostructure, environmental stability, and antioxidant activity of microemulsions containing Eucommia ulmoides Oliver seed oil is a potential delivery system as an alternative to α-linolenic acid and can be used for the delivery of peptides, proteins, antioxidants, and water-soluble nutrients.
杜仲籽油（Eucommia ulmoides Oliver seed oil，E.u oil）作为一种功能性油脂，富含多种天然活性成分，如α-亚麻酸（56%-63%）、维生素 E、梓醇等。本研究以杜仲籽油、蒸馏水、山梨醇酐单油酸酯 80（Span 80）和聚氧乙烯失水山梨醇单油酸酯（Tween 80）的混合物、丙二醇为原料，制备了 W/O 型微乳液，以提高杜仲籽油的相容性。基于亲水-亲油平衡值、助表面活性剂类型、助表面活性剂比例和环境变化应激，构建了伪三元相图来阐明微乳液的相行为。采用动态光散射、透射电子显微镜和电导率测量对微乳液的微观结构进行了表征。当丙二醇作为助表面活性剂，水-丙二醇和 Span 80-Tween 80 的比例分别保持在 1:1 和 6:4 时，载有杜仲籽油的微乳液具有良好的 pH 和盐度耐受性，是最佳的工艺条件。这些微乳液具有较窄的粒径分布、纳米级粒径（低于 60nm）、透明外观，油相含量范围较宽，对 DPPH 和 ABTS 自由基的清除能力较强，半数最大抑制浓度（IC ）值分别为 49.20 和 33.43mg/mL。实际应用：含有杜仲籽油的微乳液具有纳米结构、环境稳定性和抗氧化活性，是替代α-亚麻酸的潜在输送系统，可用于输送肽、蛋白质、抗氧化剂和水溶性营养素。
