State Key Laboratory of Component-Based Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
State Key Laboratory of Component-Based Chinese Medicine, Haihe Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Molecules. 2022 Sep 12;27(18):5924. doi: 10.3390/molecules27185924.
As a traditional Chinese medicine, Oliver ( Oliv.) is an important medicinal plant, and its barks, male flowers, leaves, and fruits have high value of utilization. The seed meal of Oliv. is the waste residue produced after oil extraction from seeds of Oliv. Though the seed meal of Oliv. is an ideal feed additive, its medicinal value is far from being developed and utilized. We identified six natural iridoid compounds from the seed meal of Oliv., namely geniposidic acid (GPA), scyphiphin D (SD), ulmoidoside A (UA), ulmoidoside B (UB), ulmoidoside C (UC), and ulmoidoside D (UD). Six natural iridoid compounds were validated to have anti-inflammatory activities. Hence, six compounds were quantified at the optimum extracting conditions in the seed meal of Oliv. by an established ultra-performance liquid chromatography (UPLC) method. Some interesting conversion phenomena of six tested compounds were uncovered by a systematic study of stability performed under different temperatures and pH levels. GPA was certified to be stable. SD, UA, and UC were only hydrolyzed under strong alkaline solution. UB and UD were affected by high temperature, alkaline, and strong acid conditions. Our findings reveal the active compounds and explore the quantitative analysis of the tested compounds, contributing to rational utilization for the seeds residues of Oliv.
作为一种传统中药，吴茱萸（Oliv.）是一种重要的药用植物，其树皮、雄花、叶和果实具有很高的利用价值。吴茱萸种子粕是从吴茱萸种子中提取油后产生的废渣。虽然吴茱萸种子粕是一种理想的饲料添加剂，但它的药用价值还远未得到开发和利用。我们从吴茱萸种子粕中鉴定出 6 种天然环烯醚萜类化合物，分别为京尼平酸（GPA）、scyphiphin D（SD）、玉兰苷 A（UA）、玉兰苷 B（UB）、玉兰苷 C（UC）和玉兰苷 D（UD）。6 种天然环烯醚萜类化合物均具有抗炎活性。因此，在吴茱萸种子粕的最佳提取条件下，采用建立的超高效液相色谱（UPLC）法对 6 种天然环烯醚萜类化合物进行了定量分析。通过对不同温度和 pH 值条件下稳定性的系统研究，发现了 6 种测试化合物的一些有趣的转化现象。GPA 被证明是稳定的。SD、UA 和 UC 仅在强碱性溶液中水解。UB 和 UD 受高温、碱性和强酸条件的影响。我们的研究结果揭示了活性化合物，并探讨了测试化合物的定量分析，为吴茱萸种子残渣的合理利用提供了依据。
