Tsuruta H, Tsubomura H
Department of Chemistry, Faculty of Engineering Science, Osaka University, Japan.
Appl Biochem Biotechnol. 1988 Nov;19(2):151-62. doi: 10.1007/BF02921480.
The method of highly selective potentiometric detection of bioactive substances developed by us has been much improved by using tantalum metal as a electrode material, by employment of a new method of modification (using gamma-aminopropyltriethoxysilane and cyanogen bromide), and by the pretreatment with inert protein before the measurement. As the consequence, the response in the electrode potential shift has been increased and the reproducibility of the data reached almost 100%.
我们开发的生物活性物质高选择性电位检测方法,通过使用钽金属作为电极材料、采用新的修饰方法(使用γ-氨丙基三乙氧基硅烷和溴化氰)以及在测量前用惰性蛋白质进行预处理,有了很大改进。结果,电极电位偏移的响应增加了,数据的重现性几乎达到了100%。