使用化学修饰金属电极对抗抗原-抗体和酶-酶抑制剂反应进行电位测定研究。

Potentiometric investigations of antigen-antibody and enzyme-enzyme inhibitor reactions using chemically modified metal electrodes.

作者信息

Yamamoto N, Nagasawa Y, Sawai M, Sudo T, Tsubomura H

出版信息

J Immunol Methods. 1978;22(3-4):309-17. doi: 10.1016/0022-1759(78)90038-8.

Abstract

The electrical potential between an immunoreactive electrode and a reference electrode in a buffer solution was studied. The immunoelectrode was made of titanium wire, on which an antigen or an antibody was chemically fixed. The electric potential of the electrode sensitized with anti-hCG gamma-globulin shifted in the positive direction in the presence of a small amount of hCG in the solution. On the other hand, the potential of the hCG-sensitized electrode ran in the negative direction upon addition of anti-hCG to the buffer solution. Similar changes in potential were observed between trypsin and its inhibitor, aprotinin. Kinetic analysis was made for the reactions between these species and the change in potential was explained by a simple charge transfer model.

摘要

研究了免疫反应电极与缓冲溶液中参比电极之间的电势。免疫电极由钛丝制成，抗原或抗体通过化学方法固定在其上。在用抗人绒毛膜促性腺激素γ球蛋白致敏的电极存在下，溶液中少量人绒毛膜促性腺激素会使电极电势向正方向移动。另一方面，向缓冲溶液中加入抗人绒毛膜促性腺激素后，人绒毛膜促性腺激素致敏电极的电势向负方向移动。在胰蛋白酶及其抑制剂抑肽酶之间也观察到了类似的电势变化。对这些物质之间的反应进行了动力学分析，电势变化通过一个简单的电荷转移模型进行了解释。