Department of Genome Sciences, BC Cancer, 675 West 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada.
Org Lett. 2020 Jun 5;22(11):4053-4057. doi: 10.1021/acs.orglett.0c00818. Epub 2020 Apr 13.
Methods for the focused isolation of low-abundance natural products with specific chemical substructures could expand known bioactive chemical diversity for drug discovery. Here we report the combined use of genome mining and an N NMR-based screening method for the targeted isolation of the low-abundance piperazic-acid-containing peptides incarnatapeptins A () and B (). Incarnatapeptin B () shows cytotoxicity to LNCaP prostate cancer cells.
方法专注于低丰度天然产物的分离，具有特定化学亚结构，可以扩大已知生物活性化学多样性的药物发现。在这里，我们报告了基因组挖掘和基于 N NMR 的筛选方法的联合使用，用于靶向分离低丰度哌嗪酸含肽 incarnatapeptins A () 和 B (). Incarnatapeptin B () 对 LNCaP 前列腺癌细胞具有细胞毒性。
