Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
J Antibiot (Tokyo). 2019 Jan;72(1):1-7. doi: 10.1038/s41429-018-0105-4. Epub 2018 Oct 11.
Using genome mining, a new cytotoxic peptide named curacozole was isolated from Streptomyces curacoi. Through ESI-MS and NMR analyses, curacozole was determined to be a macrocyclic peptide containing two isoleucine, two thiazole and three oxazole moieties. Curacozole exhibited potent cytotoxic activity against HCT116 and HOS cancer cells. The proposed biosynthetic gene cluster of curacozole was identified and compared with that of the related compound YM-216391.
利用基因组挖掘技术，从链霉菌属中分离得到一种新型细胞毒性肽，命名为库拉佐尔。通过 ESI-MS 和 NMR 分析，确定库拉佐尔是一种含有两个异亮氨酸、两个噻唑和三个噁唑部分的大环肽。库拉佐尔对 HCT116 和 HOS 癌细胞表现出强烈的细胞毒性。鉴定了库拉佐尔的生物合成基因簇，并与相关化合物 YM-216391 的基因簇进行了比较。
