Vlasov L G, Tolstykh P I, Ignatiuk T E, Razzakov O N
Antibiot Khimioter. 1988 Nov;33(11):848-50.
Immobilized forms of lysozyme were prepared by its covalent binding on dialdehyde cellulose and polycaproamide fibres as woven and knitted fabrics respectively. The preparations were estimated by the content of protein and bacteriolytic activity. The lysozyme activity per 1 g of the carrier and the protein content on dialdehyde cellulose were several times higher than those on polycaproamide while the specific activity of lysozyme on the polycaproamide carrier was somewhat higher than that on dialdehyde cellulose. The effect of the immobilized lysozyme in treatment of purulent wounds was studied on albino rats. It was shown that the periods of the wound healing with the use of the immobilized lysozyme were shorter than those with the use of native lysozyme. Cytological and morphological investigation of the wound wall confirmed the higher efficacy of the lysozyme immobilized forms in treatment of purulent wounds as compared to the use of the native enzyme.
通过将溶菌酶分别共价结合到二醛纤维素和聚己内酰胺纤维（机织物和针织物）上制备了固定化形式的溶菌酶。通过蛋白质含量和溶菌活性对制剂进行评估。每1克载体上的溶菌酶活性以及二醛纤维素上的蛋白质含量比聚己内酰胺上的高出几倍，而聚己内酰胺载体上溶菌酶的比活性略高于二醛纤维素上的。在白化大鼠上研究了固定化溶菌酶治疗化脓性伤口的效果。结果表明，使用固定化溶菌酶时伤口愈合的时间比使用天然溶菌酶时短。伤口壁的细胞学和形态学研究证实，与使用天然酶相比，固定化溶菌酶形式在治疗化脓性伤口方面具有更高的疗效。
