黄芩素对 Saos-2 骨肉瘤细胞系中 Wnt/β-catenin 通路和 miR-25 表达的影响。
The effect of baicalein on Wnt/β-catenin pathway and miR-25 expression in Saos-2 osteosarcoma cell line.
机构信息
Department of Nutrition and Dietetics, Faculty of Health Sciences, Aydın Adnan Menderes University, Aydın, Turkey
Department of Biology, Faculty of Arts and Science, Aydın Adnan Menderes University, Aydın, Turkey
出版信息
Turk J Med Sci. 2020 Jun 23;50(44):1168-1179. doi: 10.3906/sag-2001-161.
BACKGROUND/AIM: Osteosarcoma is the most common primary bone malignancy that occurs frequently in children and adolescents. Baicalein, a flavonoid that has attracted great attention in recent years with its strong antitumor activity, shows a wide range of biological and pharmaceutical effects.MicroRNAs have been found to be involved in many critical processes in cancers. This study aimed to investigate the effect of baicalein and miR-25 on Wnt/β-catenin signaling pathway of osteosarcoma cell line Saos-2.
MATERIALS AND METHODS
Cell viability was assessed, and qRT-PCR and Western blot were performed to study the effects of baicalein on expression of Wnt/β-catenin signaling pathway-realted genes (β-catenin, GSK-3β, and Axin2) of Saos-2 cells.
RESULTS
Our results indicated that baicalein can inhibit the proliferation (IC50 value 35 μM), regulate Wnt/β-catenin pathway and also increase miR-25 expression of Saos-2. Baicalein and also miR-25 decreased the expression of β-catenin and Axin2, while increasing the expression of GSK-3β. Down regulation of miR-25 decreased the expression of GSK-3β, while β-catenin and Axin2 expression increased.
CONCLUSION
These findings demonstrate that baicalein may target genes related to the Wnt/β-catenin pathway by regulating miR-25 expression and may be a potential Wnt/β-catenin pathway inhibitor for osteosarcoma therapy.
背景/目的：骨肉瘤是最常见的原发性骨恶性肿瘤，好发于儿童和青少年。近年来，由于其强大的抗肿瘤活性，黄芩素作为一种黄酮类化合物受到了极大的关注，表现出广泛的生物学和药物学作用。微小 RNA 已被发现参与癌症的许多关键过程。本研究旨在探讨黄芩素和 miR-25 对骨肉瘤细胞系 Saos-2 中 Wnt/β-catenin 信号通路的影响。
材料和方法
通过细胞活力评估和 qRT-PCR 和 Western blot 检测，研究黄芩素对 Saos-2 细胞 Wnt/β-catenin 信号通路相关基因（β-catenin、GSK-3β和 Axin2）表达的影响。
结果
我们的结果表明，黄芩素可以抑制增殖（IC50 值为 35 μM），调节 Wnt/β-catenin 通路，增加 Saos-2 中 miR-25 的表达。黄芩素和 miR-25 降低了β-catenin 和 Axin2 的表达，同时增加了 GSK-3β的表达。下调 miR-25 降低了 GSK-3β的表达，而β-catenin 和 Axin2 的表达增加。
结论
这些发现表明，黄芩素可能通过调节 miR-25 的表达来靶向与 Wnt/β-catenin 通路相关的基因，可能是骨肉瘤治疗中潜在的 Wnt/β-catenin 通路抑制剂。
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