Hevelone J, Hartman P S
Department of Biology, Texas Christian University, Forth Worth 76129.
Biochem Genet. 1988 Aug;26(7-8):447-61. doi: 10.1007/BF02399412.
A deoxyribonuclease was partially purified from the free-living nematode Caenorhabditis elegans. The DNase functioned as an endonuclease and introduced both single-strand nicks and double-strand breaks into DNA. The enzyme hydrolyzed double-stranded DNA seven times more rapidly than single-stranded DNA. DNase activity was not affected by the addition of divalent cations below 1 mM but was inhibited at higher ionic concentrations. In addition, the enzyme was not inhibited in the presence of 10 mM EDTA. The enzyme was inhibited by salt concentrations greater than 20 mM. Three independent mutations in the nuc-1 gene were shown to reduce nuclease activity to less than 1% of that seen in wild-type organisms.
从自由生活的线虫秀丽隐杆线虫中部分纯化出一种脱氧核糖核酸酶。该脱氧核糖核酸酶作为一种核酸内切酶发挥作用，能在DNA中引入单链切口和双链断裂。该酶水解双链DNA的速度比单链DNA快7倍。低于1 mM的二价阳离子添加不会影响脱氧核糖核酸酶的活性，但在较高离子浓度下会受到抑制。此外，在10 mM乙二胺四乙酸（EDTA）存在的情况下该酶不会被抑制。盐浓度大于20 mM时该酶会受到抑制。nuc-1基因中的三个独立突变显示可将核酸酶活性降低至野生型生物体中所见活性的1%以下。
