Osterman J C
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
Biochem Genet. 1988 Aug;26(7-8):463-74. doi: 10.1007/BF02399413.
An allele of the Prot locus, which encodes a major globulin of the maize scutellum, is a variant for a site of protein processing. Segregation analysis and recombination mapping indicate that the variant is an allele of the Prot locus. Designated Prot-V, this allele specifies three polypeptides, V1, V2, and V3. The V1 polypeptide is incompletely processed during the proteolytic processing step catalyzed by the product of the Mep locus. Cyanogen bromide cleavage studies support the precursor-product relationship between V1 and V2. The V1 product is shortened with respect to other PROT' proteins and it is postulated that the normal site of MEP processing has been removed by this foreshortening.
编码玉米盾片中一种主要球蛋白的Prot基因座的一个等位基因,是蛋白质加工位点的一个变体。分离分析和重组作图表明该变体是Prot基因座的一个等位基因。这个等位基因被命名为Prot-V,它指定了三种多肽,即V1、V2和V3。在由Mep基因座产物催化的蛋白水解加工步骤中,V1多肽未被完全加工。溴化氰裂解研究支持V1和V2之间的前体-产物关系。相对于其他PROT'蛋白,V1产物有所缩短,据推测,MEP加工的正常位点已因这种缩短而被去除。