Grüßer Linda, Blaumeiser-Debarry Rosmarie, Rossaint Rolf, Krings Matthias, Kremer Benedikt, Höllig Anke, Coburn Mark
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Department of Anesthesiology and Intensive Care, Medizinisches Zentrum StaedteRegion Aachen, Aachen, Germany.
Basic Clin Neurosci. 2019 Sep-Oct;10(5):485-498. doi: 10.32598/bcn.9.10.235. Epub 2019 Sep 1.
Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a setup. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied.
We defined necessary checkpoints and developed inclusion and exclusion criteria that take the observed variation in such a model into consideration. Objective measures include the identification and exclusion of pre-damaged slices and outliers. Six steps were outlined in this study.
A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that through equal requirements set for all slices more precise findings are ensured.
Results in a TBI experiment on OHBS should be analyzed critically as inhomogeneities may occur. In order to ensure more precise findings, a structured approach of comparing the results should be followed. Further research is recommended to confirm and further develop this framework.
器官型海马脑片（OHBS）为研究创伤性脑损伤（TBI）提供了一种优于体内模型的替代方法。我们遵循了一种成熟的TBI方案，但注意到在这种设置中，有几个因素可能会影响结果。在这里，我们描述了一种结构化方法，以产生更具可比性的结果，并讨论了为何应应用特定的纳入标准。
我们定义了必要的检查点，并制定了纳入和排除标准，将在这种模型中观察到的变异性考虑在内。客观测量包括识别和排除预先受损的脑片和异常值。本研究概述了六个步骤。
提出了一种增强可比性的六步法，并以流程图形式进行了总结。我们将建议的措施应用于从我们的TBI实验获得的数据，该实验研究了三种不同干预措施对1459个OHBS的影响。我们的示例结果表明，通过对所有脑片设定相同的要求，可以确保更精确的结果。
在OHBS上进行的TBI实验结果应进行批判性分析，因为可能会出现不均匀性。为了确保更精确的结果，应遵循一种比较结果的结构化方法。建议进一步开展研究以证实并进一步完善该框架。
